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Yang Sheng
Professor
Institute of Plant Physiology and Ecology
86-21-54924173
: syang@sibs.ac.cn

Research Focus

My research directions are metabolic engineering of industrial microorganisms such as Clostridia, Escherichia coli, Bacillus subtilis and yeast; protein engineering of industrial enzymes; development and application of new technologies for engineering genes, proteins, pathways and genomes. The goal for these work is to develop and optimize genetically-based bioproducts and bioprocesses.

 

Biography

Education

1995-2000 Ph.D., Biochemistry and Molecular Biology, Shanghai Institute of Biochemistry, Chinese Academy of Sciences                                                         

1991-1995 B.E., Chemical Engineering, Zhejiang University, Hangzhou, China.                   

Academic Experience

2008-present  Director, Shanghai Research and Development Center of Industrial Biotechnology       

2007-present  Assistant Director, Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences                                                         

2006-present  Director, Huzhou Research Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences                                                         

2006-present  Professor of Microbiology, Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences                                                         

2002-2006 Associate Professor of Microbiology, Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences                                            

2000-2002 Assistant Professor of Biochemistry, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences            


Selected Publications

1. Yang GU, Sheng YANG*, Weihong JIANG*, Improvement of xylose utilization in Clostridium acetobutylicum via expression of the talA gene encoding transaldolase from Escherichia coli. Journal of Biotechnology, accepted
2. Yu JIANG, Chongmao XU, Feng DONG, Yunliu YANG, Weihong JIANG*, Sheng YANG* (2009) Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio, Metabolic Engineering,11, 284-91
3. Yang GU, Shiyuan HU, Jun CHEN, Lijun SHAO, Huiqi HE, Yunliu YANG, Sheng YANG*, Weihong JIANG*. Ammonium acetate enhances solvent production by Clostridium acetobutylicum EA 2018 using cassava as a fermentation medium. J Ind Microbiol Biotechnol, DOI: 10.1007/s10295-009-0604-1.
4. Junjie YANG, Weihong JIANG, Sheng YANG* (2009). mazF as a counter-selectable marker for unmarked genetic modification of Pichia pastoris. FEMS Yeast Research. 9(4): 600-9

5. Hong YU, Jian LI, Dalong ZHANG, Yunliu YANG, Weihong JIANG, Sheng YANG*. (2009) Improving the thermostability of N-carbamyl-D-amino acid amidohydrolase by error-prone PCR. Appl. Microbiol. Biotechnol. 82(2): 279-85

6. Lijun SHAO, Shiyuan HU, Yi YANG, Yang GU, Jun CHEN, Yunliu YANG, Weihong JIANG*, Sheng YANG* (2007) Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum. Cell Research. 17: 963-5

7. Huabao ZHENG, Jun CHEN, Liuli SU, Yuhua ZHAO, Yunliu YANG, Sheng YANG* and Weihong JIANG (2007) One-step purification and immobilization of GL-7-ACA acylase by affinity resins. Enzymatic and Microbial Technology. 41: 474-9.

8. Jingang WANG, Qing ZHANG, He HUANG, Zhongyi YUAN, Dafu DING, Sheng YANG*, Weihong JIANG (2007) Increasing Synthetic Performance of Penicillin G Acylase from Bacillus megaterium by Site-directed Mutagenesis. Applied Microbiology and Biotechnology. 74: 1023-30.

9. Huabao ZHENG, Xiaolan WANG, Jun CHEN, Ke ZHU, Yuhua ZHAO, Yunliu YANG, Sheng YANG*, Weihong JIANG (2006) Expression, purification and immobilization of His-tagged D-amino acid oxidase of Trigonopsis variabilis in Pichia pastoris, Applied Microbiology and Biotechnology, 70(6): 683-9

10. Tianfan CHENG, Maolin CHEN, Huabao ZHENG, Jingang WANG, Sheng YANG*, Weihong JIANG (2006) Expression and Purification of Penicillin G Acylase Enzymes from Four Different Micro-organisms, and a Comparative Evaluation of Their Synthesis/Hydrolysis Ratios for Cephalexin. Protein Expression and Purification, 46, 107-13

11. Yu XIA, Wei CHEN, Sheng YANG*, Xiaoyan FU, Hao ZHANG*, Xiaolin DING (2005) Construction of an integrative food-grade expression system for Bacillus subtilis. Food Research International. 38(3), 251-6

12. Li PENG, Zhinan XU*, Xiangming FANG, Fang WANG, Sheng YANG*, Peilin CEN (2004) Preferential codons enhancing the expression level of human β-defensin-2 in recombinant Escherichia coli. Protein and Peptide Letters  11(4), 339-44

13. Xiaoli ZHANG, Jingru WANG, Yan ZHANG, Maolin CHEN, Wei ZHANG, Sheng YANG*, Weihong JIANG* (2003) Expression, Purification and Identification of Recombinant SARS Coronavirus Membrane Protein. Acta Biochimica et Biophysica Sinica 35(12): 1140-4

14. Hao YU, Yong YANG, Wei ZHANG, Youhua XIE, Jun QIN, Yuan WANG, Huabao ZHENG, Guoping ZHAO, Sheng YANG*, Weihong JIANG* (2003) Expression and purification of recombinant SARS coronavirus spike protein. ACTA BIOCHIMICA et BIOPHYSICA SINICA 35(8):774-8

15. Zheng ZHOU, Aihui ZHANG, Jingru WANG, Maolin CHEN, Renbao LI, Sheng YANG*, Zhongyi YUAN * (2003) Improving the specific synthetic activity of a penicillin G acylase using DNA family shuffling. ACTA BIOCHIMICA et BIOPHYSICA SINICA 35(6):573-9

16. Sheng YANG, Haixu TANG, Jiang PAN, Xingjia WU, Lu TIAN, He HUANG, Zhongyi YUAN* (2002) Rational design of penicillin G acylase with high stability. Journal of Molecular Catalysis B: Enzymatic 18(4-6), 279-284

 

17. Sheng YANG, He HUANG, Ruan ZHANG, Xiaodong HUANG, Shiyun LI, and Zhongyi YUAN* (2001) Expression and Purification of Extracellular Penicillin G Acylase in Bacillus subtilis. Protein Expression and Purification 21, 60–64

 
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