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论文题目: Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System
英文论文题目: Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System
第一作者: Jiang, Y; Chen, B; Duan, CL; Sun, BB; Yang, JJ; Yang, S
英文第一作者: Jiang, Y; Chen, B; Duan, CL; Sun, BB; Yang, JJ; Yang, S
联系作者: Yang, S (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai, Peoples R China.
英文联系作者: Yang, S (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai, Peoples R China.
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发表年度: 2015
卷: 81
期: 7
页码: 2506-2514
摘要: An efficient genome-scale editing tool is required for construction of industrially useful microbes. We describe a targeted, continual multigene editing strategy that was applied to the Escherichia coli genome by using the Streptococcus pyogenes type II CRISPR-Cas9 system to realize a variety of precise genome modifications, including gene deletion and insertion, with a highest efficiency of 100%, which was able to achieve simultaneous multigene editing of up to three targets. The system also demonstrated successful targeted chromosomal deletions in Tatumella citrea, another species of the Enterobacteriaceae, with highest efficiency of 100%.
英文摘要: An efficient genome-scale editing tool is required for construction of industrially useful microbes. We describe a targeted, continual multigene editing strategy that was applied to the Escherichia coli genome by using the Streptococcus pyogenes type II CRISPR-Cas9 system to realize a variety of precise genome modifications, including gene deletion and insertion, with a highest efficiency of 100%, which was able to achieve simultaneous multigene editing of up to three targets. The system also demonstrated successful targeted chromosomal deletions in Tatumella citrea, another species of the Enterobacteriaceae, with highest efficiency of 100%.
刊物名称: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
英文刊物名称: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
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学科: Biotechnology & Applied Microbiology; Microbiology
英文学科: Biotechnology & Applied Microbiology; Microbiology
影响因子: 3.668
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论文类别: Article
英文论文类别: Article
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