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论文题目: Altered microRNAs expression profiling in cumulus cells from patients with polycystic ovary syndrome
英文论文题目: Altered microRNAs expression profiling in cumulus cells from patients with polycystic ovary syndrome
第一作者: Liu, SY; Zhang, X; Shi, CG; Lin, JM; Chen, GW; Wu, B; Wu, LG; Shi, HJ; Yuan, Y; Zhou, WJ; Sun, ZG; Dong, X; Wang, J
英文第一作者: Liu, SY; Zhang, X; Shi, CG; Lin, JM; Chen, GW; Wu, B; Wu, LG; Shi, HJ; Yuan, Y; Zhou, WJ; Sun, ZG; Dong, X; Wang, J
联系作者: Zhang, X (reprint author), SIPPR, Natl Populat & Family Planning Key Lab Contracept, Shanghai, Peoples R China.
英文联系作者: Zhang, X (reprint author), SIPPR, Natl Populat & Family Planning Key Lab Contracept, Shanghai, Peoples R China.
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发表年度: 2015
卷: 13
期:
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摘要: Background: Polycystic ovary syndrome (PCOS) is a common endocrine disorder in women of reproductive age, and oocyte developmental competence is altered in patients with PCOS. In recent years microRNAs (miRNAs) have emerged as important regulators of gene expression, the aim of the study was to study miRNAs expression patterns of cumulus cells from PCOS patients. Methods: The study included 20 patients undergoing in vitro fertilization (IVF) and intra-cytoplasmic sperm injection (ICSI): 10 diagnosed with PCOS and 10 matching controls. We used deep sequencing technology to identify the miRNAs differentially expressed in the cumulus cells of PCOS. Results: There were 17 differentially expressed miRNAs in PCOS cumulus cells, including 10 miRNAs increase and 7 miRNAs decrease. These miRNAs were predicted to target a large set of genes with different functions, including Wnt- and MAPK-signaling pathways, oocyte meiosis, progesterone-mediated oocyte maturation and cell cycle. Unsupervised hierarchical clustering analysis demonstrated that there was a specific miRNAs expression pattern in PCOS cumulus cells. Conclusion: We found that the miRNAs expression profile was different in cumulus cells isolated from PCOS patients compared with control. This study provided new evidence for understanding the pathogenesis of PCOS.
英文摘要: Background: Polycystic ovary syndrome (PCOS) is a common endocrine disorder in women of reproductive age, and oocyte developmental competence is altered in patients with PCOS. In recent years microRNAs (miRNAs) have emerged as important regulators of gene expression, the aim of the study was to study miRNAs expression patterns of cumulus cells from PCOS patients. Methods: The study included 20 patients undergoing in vitro fertilization (IVF) and intra-cytoplasmic sperm injection (ICSI): 10 diagnosed with PCOS and 10 matching controls. We used deep sequencing technology to identify the miRNAs differentially expressed in the cumulus cells of PCOS. Results: There were 17 differentially expressed miRNAs in PCOS cumulus cells, including 10 miRNAs increase and 7 miRNAs decrease. These miRNAs were predicted to target a large set of genes with different functions, including Wnt- and MAPK-signaling pathways, oocyte meiosis, progesterone-mediated oocyte maturation and cell cycle. Unsupervised hierarchical clustering analysis demonstrated that there was a specific miRNAs expression pattern in PCOS cumulus cells. Conclusion: We found that the miRNAs expression profile was different in cumulus cells isolated from PCOS patients compared with control. This study provided new evidence for understanding the pathogenesis of PCOS.
刊物名称: JOURNAL OF TRANSLATIONAL MEDICINE
英文刊物名称: JOURNAL OF TRANSLATIONAL MEDICINE
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学科: Research & Experimental Medicine
英文学科: Research & Experimental Medicine
影响因子: 3.93
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论文类别: Article
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