论文库首页  论文库
 
论文编号:
论文题目: Phytosulfokine Is Involved in Positive Regulation of Lotus japonicus Nodulation
英文论文题目: Phytosulfokine Is Involved in Positive Regulation of Lotus japonicus Nodulation
第一作者: Wang, C; Yu, HX; Zhang, ZM; Yu, LL; Xu, XS; Hong, ZL; Luo, L
英文第一作者: Wang, C; Yu, HX; Zhang, ZM; Yu, LL; Xu, XS; Hong, ZL; Luo, L
联系作者: Luo, L (reprint author), Shanghai Univ, Sch Life Sci, Shanghai Key Lab Bioenergy Crops, Shanghai 200444, Peoples R China.
英文联系作者: Luo, L (reprint author), Shanghai Univ, Sch Life Sci, Shanghai Key Lab Bioenergy Crops, Shanghai 200444, Peoples R China.
外单位作者单位:
英文外单位作者单位:
发表年度: 2015
卷: 28
期: 8
页码: 847-855
摘要: Phytosulfokine (PSK) is a tyrosine-sulfated peptide that is widely distributed in plants, participating in cell proliferation, differentiation, and innate immunity. The potential role of PSK in nodulation in legumes has not been reported. In this work, five PSK precursor genes were identified in Lotus japonicas, designated as LjPSK1 to LjPSK5. Three of them (LjPSK1, LjPSK4, and LjPSK5) were found to be expressed in nitrogen-fixing root nodules. LjPSK1 and LjPSK4 were not induced at the early stage of nodulation. Interestingly, while the expression of LjPSK4 was also found in spontaneous nodules without rhizobial colonization, LjPSK1 was not induced in these pseudo nodules. Promoter-beta-glucuronidase analysis revealed that LjPSK1 was highly expressed in enlarged symbiotic cells of nodules. Exogenous addition of 1 1M synthetic PSK peptide resulted in increased nodule numbers per plant. Consistently, the number of mature nodules but not the events of rhizobial infection and nodule initiation was increased by overexpressing LjPSK1 in transgenic hairy roots, in which the expression of jasmonate-responsive genes was found to be repressed. These results suggest that PSK is a new peptide signal that regulates nodulation in legumes, probably through cross-talking with other phytohormones.
英文摘要: Phytosulfokine (PSK) is a tyrosine-sulfated peptide that is widely distributed in plants, participating in cell proliferation, differentiation, and innate immunity. The potential role of PSK in nodulation in legumes has not been reported. In this work, five PSK precursor genes were identified in Lotus japonicas, designated as LjPSK1 to LjPSK5. Three of them (LjPSK1, LjPSK4, and LjPSK5) were found to be expressed in nitrogen-fixing root nodules. LjPSK1 and LjPSK4 were not induced at the early stage of nodulation. Interestingly, while the expression of LjPSK4 was also found in spontaneous nodules without rhizobial colonization, LjPSK1 was not induced in these pseudo nodules. Promoter-beta-glucuronidase analysis revealed that LjPSK1 was highly expressed in enlarged symbiotic cells of nodules. Exogenous addition of 1 1M synthetic PSK peptide resulted in increased nodule numbers per plant. Consistently, the number of mature nodules but not the events of rhizobial infection and nodule initiation was increased by overexpressing LjPSK1 in transgenic hairy roots, in which the expression of jasmonate-responsive genes was found to be repressed. These results suggest that PSK is a new peptide signal that regulates nodulation in legumes, probably through cross-talking with other phytohormones.
刊物名称: MOLECULAR PLANT-MICROBE INTERACTIONS
英文刊物名称: MOLECULAR PLANT-MICROBE INTERACTIONS
论文全文:
英文论文全文:
全文链接:
其它备注:
英文其它备注:
学科: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences
英文学科: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences
影响因子: 3.944
第一作者所在部门:
英文第一作者所在部门:
论文出处:
英文论文出处:
论文类别: Article
英文论文类别: Article
参与作者:
英文参与作者:
 
2014 中国科学院上海生命科学研究院 版权所有