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论文题目: Curcumin suppresses invasiveness and vasculogenic mimicry of squamous cell carcinoma of the larynx through the inhibition of JAK-2/STAT-3 signaling pathway
英文论文题目: Curcumin suppresses invasiveness and vasculogenic mimicry of squamous cell carcinoma of the larynx through the inhibition of JAK-2/STAT-3 signaling pathway
第一作者: Hu, A; Huang, JJ; Jin, XJ; Li, JP; Tang, YJ; Huang, XF; Cui, HJ; Xu, WH; Sun, GB
英文第一作者: Hu, A; Huang, JJ; Jin, XJ; Li, JP; Tang, YJ; Huang, XF; Cui, HJ; Xu, WH; Sun, GB
联系作者: Huang, JJ (reprint author), Shanghai Jiao Tong Univ, Sch Med, Dept Gerontol, Xinhua Hosp, Kongjiang Rd 1665, Shanghai 200092, Peoples R China.
英文联系作者: Huang, JJ (reprint author), Shanghai Jiao Tong Univ, Sch Med, Dept Gerontol, Xinhua Hosp, Kongjiang Rd 1665, Shanghai 200092, Peoples R China.
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发表年度: 2015
卷: 5
期: 1
页码: 278-288
摘要: To determine the role of JAK-2/STAT-3 signaling pathway in invasion and vasculogenic mimicry of laryngeal squamous cell carcinoma. HEp-2 cells were treated with 1 or 10 mu mol/L curcumin and AG490 (the inhibitor of JAK-2) for 48 h, the invasion and vasculogenic mimicry of tumor cells were tested with Transwell chamber test and tube formation experiment. RT-PCR was used to measure the expression of MMP-2 and VEGF. Western blot assay was employed to determine the expression of JAK-2, STAT3, p-STAT3, MMP-2 and VEGF. Compared to control group, there were less tumor cells permeating membrane and less formed tubes after curcumin or AG490 treatment, RT-PCR showed that the expression of MMP-2 and VEGF at mRNA level were decreased (P < 0.01). Western blotting indicated that the expression of JAK-2, p-STAT3, MMP-2 and VEGF at protein levels were decreased (P < 0.01), while that of STAT-3 protein had no difference among each group (P > 0.05). Immunofluorescence staining demonstrated that the expression of eNOS was down-regulated (P < 0.01). Curcumin and AG490 significantly inhibits invasion and vasculogenic mimicry of laryngeal squamous cell carcinoma in vitro, and JAK-2/STAT-3 signaling pathway promotes above processes.
英文摘要: To determine the role of JAK-2/STAT-3 signaling pathway in invasion and vasculogenic mimicry of laryngeal squamous cell carcinoma. HEp-2 cells were treated with 1 or 10 mu mol/L curcumin and AG490 (the inhibitor of JAK-2) for 48 h, the invasion and vasculogenic mimicry of tumor cells were tested with Transwell chamber test and tube formation experiment. RT-PCR was used to measure the expression of MMP-2 and VEGF. Western blot assay was employed to determine the expression of JAK-2, STAT3, p-STAT3, MMP-2 and VEGF. Compared to control group, there were less tumor cells permeating membrane and less formed tubes after curcumin or AG490 treatment, RT-PCR showed that the expression of MMP-2 and VEGF at mRNA level were decreased (P < 0.01). Western blotting indicated that the expression of JAK-2, p-STAT3, MMP-2 and VEGF at protein levels were decreased (P < 0.01), while that of STAT-3 protein had no difference among each group (P > 0.05). Immunofluorescence staining demonstrated that the expression of eNOS was down-regulated (P < 0.01). Curcumin and AG490 significantly inhibits invasion and vasculogenic mimicry of laryngeal squamous cell carcinoma in vitro, and JAK-2/STAT-3 signaling pathway promotes above processes.
刊物名称: AMERICAN JOURNAL OF CANCER RESEARCH
英文刊物名称: AMERICAN JOURNAL OF CANCER RESEARCH
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学科: Oncology
英文学科: Oncology
影响因子: 4.165
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论文类别: Article
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