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论文题目: A reliable liquid chromatography-tandem mass spectrometry method for simultaneous determination of multiple mycotoxins in fresh fish and dried seafoods
英文论文题目: A reliable liquid chromatography-tandem mass spectrometry method for simultaneous determination of multiple mycotoxins in fresh fish and dried seafoods
第一作者: Sun, WS; Han, Z; Aerts, J; Nie, D; Jin, MT; Shi, W; Zhao, ZY; De Saeger, S; Zhao, Y; Wu, AB
英文第一作者: Sun, WS; Han, Z; Aerts, J; Nie, D; Jin, MT; Shi, W; Zhao, ZY; De Saeger, S; Zhao, Y; Wu, AB
联系作者: Wu, AB (reprint author), Chinese Acad Sci, Inst Nutr Sci, Shanghai Inst Biol Sci, Key Lab Food Safety Res, 294 Taiyuan Rd, Shanghai 200031, Peoples R China.
英文联系作者: Wu, AB (reprint author), Chinese Acad Sci, Inst Nutr Sci, Shanghai Inst Biol Sci, Key Lab Food Safety Res, 294 Taiyuan Rd, Shanghai 200031, Peoples R China.
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发表年度: 2015
卷: 1387
期:
页码: 42-48
摘要: A reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of nine mycotoxins, i.e., aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2), ochratoxin A (OTA), zearalenone (ZEN), T-2 toxin, HT2 toxin and deoxynivalenol (DON), in fresh fish (muscle and entrails) as well as dried seafoods. Special focus was given to sample pretreatment which is crucial for an accurate and reliable analytical method. With regards to the high complexity of the matrices, extraction solvent, time, and temperature as well as clean-up cartridges were optimized to improve extraction efficiency and reduce matrix effects. The optimum procedure included ultrasound-assisted extraction with acetonitrile/water/acetic acid (79/20/1, v/v/v) at 40 degrees C for 30 min, defatting with n-hexane and purification by Oasis HLB cartridges. The method was further validated by determining the linearity (R-2 >= 0.9989), sensitivity (limit of detection <= 2 mu g/kg, limit of quantitation <= 3 mu g/kg), recovery (72.2-119.9%) and precision (<= 18.3%) in muscle and entrails of fresh crucian carp (Carassius carassius) as well as dried fish products. The method was proven to be suitable for its intended purposes. Mycotoxins of OTA, ZEN and AFB2 have been found in fresh fish and dried seafoods for the first time. (C) 2015 Elsevier B.V. All rights reserved.
英文摘要: A reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of nine mycotoxins, i.e., aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2), ochratoxin A (OTA), zearalenone (ZEN), T-2 toxin, HT2 toxin and deoxynivalenol (DON), in fresh fish (muscle and entrails) as well as dried seafoods. Special focus was given to sample pretreatment which is crucial for an accurate and reliable analytical method. With regards to the high complexity of the matrices, extraction solvent, time, and temperature as well as clean-up cartridges were optimized to improve extraction efficiency and reduce matrix effects. The optimum procedure included ultrasound-assisted extraction with acetonitrile/water/acetic acid (79/20/1, v/v/v) at 40 degrees C for 30 min, defatting with n-hexane and purification by Oasis HLB cartridges. The method was further validated by determining the linearity (R-2 >= 0.9989), sensitivity (limit of detection <= 2 mu g/kg, limit of quantitation <= 3 mu g/kg), recovery (72.2-119.9%) and precision (<= 18.3%) in muscle and entrails of fresh crucian carp (Carassius carassius) as well as dried fish products. The method was proven to be suitable for its intended purposes. Mycotoxins of OTA, ZEN and AFB2 have been found in fresh fish and dried seafoods for the first time. (C) 2015 Elsevier B.V. All rights reserved.
刊物名称: JOURNAL OF CHROMATOGRAPHY A
英文刊物名称: JOURNAL OF CHROMATOGRAPHY A
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学科: Biochemistry & Molecular Biology; Chemistry
英文学科: Biochemistry & Molecular Biology; Chemistry
影响因子: 4.169
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论文类别: Article
英文论文类别: Article
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