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论文题目: A simple sample pretreatment method for multi-mycotoxin determination in eggs by liquid chromatography tandem mass spectrometry
英文论文题目: A simple sample pretreatment method for multi-mycotoxin determination in eggs by liquid chromatography tandem mass spectrometry
第一作者: Zhu, RY; Zhao, ZY; Wang, JH; Bai, B; Wu, AB; Yan, LP; Song, SQ
英文第一作者: Zhu, RY; Zhao, ZY; Wang, JH; Bai, B; Wu, AB; Yan, LP; Song, SQ
联系作者: Song, SQ (reprint author), Nanjing Agr Univ, Coll Vet Med, 1 Weigang St, Nanjing 210095, Jiangsu, Peoples R China.
英文联系作者: Song, SQ (reprint author), Nanjing Agr Univ, Coll Vet Med, 1 Weigang St, Nanjing 210095, Jiangsu, Peoples R China.
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发表年度: 2015
卷: 1417
期:
页码: 1-7
摘要: In this study, a reliable and fast method using a quick, easy, cheap, effective, rugged, and safe (QuECh-ERS) extraction procedure without any clean-up step was developed for simultaneous extraction of 15 mycotoxins, i.e., aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, aflatoxin M1, aflatoxin M2, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, de-epoxy-DON, zearalenone, alpha-zearalenol, beta-zearalenol, alpha-zearalanol, and beta-zearalanol, from eggs. High-performance liquid chromatography tandem mass spectrometry was used to separate and detect all of the analytes. Electrospray ionization at both negative and positive modes and multiple reaction-monitoring mode were applied to detect these analytes. The main factors, such as extraction time, extraction solvent, evaporation temperature, and pH of the solvent, were carefully optimized to improve the extraction efficiency. The coefficients of determination of the calibration curves ranged from 0.9884 to 0.9998. The recoveries of most of the analytes were between 71.3% and 105.4% at three concentration levels, except for AFB1 that showed recovery rates of not more than 67.5% in all concentrations. The repeatability and intra-lab reproducibility of this method were both lower than 15% and 25%, respectively. The limit of quantification ranged from 0.2 mu g/kg to 5 mu g/kg. The matrix effect was evaluated and reduced by the use of matrix-matched calibration curves. The validated method was applied in a pilot study to analyze mycotoxin contamination in 12 eggs, and trace amounts of deoxynivalenol, 15-acetyldeoxynivalenol, aflatoxin B1, aflatoxin G2, zearalenone and beta-zearalenol were detected in these samples. (C) 2015 Elsevier B.V. All rights reserved.
英文摘要: In this study, a reliable and fast method using a quick, easy, cheap, effective, rugged, and safe (QuECh-ERS) extraction procedure without any clean-up step was developed for simultaneous extraction of 15 mycotoxins, i.e., aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, aflatoxin M1, aflatoxin M2, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, de-epoxy-DON, zearalenone, alpha-zearalenol, beta-zearalenol, alpha-zearalanol, and beta-zearalanol, from eggs. High-performance liquid chromatography tandem mass spectrometry was used to separate and detect all of the analytes. Electrospray ionization at both negative and positive modes and multiple reaction-monitoring mode were applied to detect these analytes. The main factors, such as extraction time, extraction solvent, evaporation temperature, and pH of the solvent, were carefully optimized to improve the extraction efficiency. The coefficients of determination of the calibration curves ranged from 0.9884 to 0.9998. The recoveries of most of the analytes were between 71.3% and 105.4% at three concentration levels, except for AFB1 that showed recovery rates of not more than 67.5% in all concentrations. The repeatability and intra-lab reproducibility of this method were both lower than 15% and 25%, respectively. The limit of quantification ranged from 0.2 mu g/kg to 5 mu g/kg. The matrix effect was evaluated and reduced by the use of matrix-matched calibration curves. The validated method was applied in a pilot study to analyze mycotoxin contamination in 12 eggs, and trace amounts of deoxynivalenol, 15-acetyldeoxynivalenol, aflatoxin B1, aflatoxin G2, zearalenone and beta-zearalenol were detected in these samples. (C) 2015 Elsevier B.V. All rights reserved.
刊物名称: JOURNAL OF CHROMATOGRAPHY A
英文刊物名称: JOURNAL OF CHROMATOGRAPHY A
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学科: Biochemistry & Molecular Biology; Chemistry
英文学科: Biochemistry & Molecular Biology; Chemistry
影响因子: 4.169
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论文类别: Article
英文论文类别: Article
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