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论文题目: A novel three-component system-based regulatory model for D-xylose sensing and transport in Clostridium beijerinckii
英文论文题目: A novel three-component system-based regulatory model for D-xylose sensing and transport in Clostridium beijerinckii
第一作者: Sun, Z; Chen, YX; Yang, C; Yang, S; Gu, Y; Jiang, WH
英文第一作者: Sun, Z; Chen, YX; Yang, C; Yang, S; Gu, Y; Jiang, WH
联系作者: Gu, Y (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai 200032, Peoples R China.
英文联系作者: Gu, Y (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai 200032, Peoples R China.
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发表年度: 2015
卷: 95
期: 4
页码: 576-589
摘要: d-Xylose is the most abundant fermentable pentose in nature and can serve as a carbon source for many bacterial species. Since d-xylose constitutes the major component of hemicellulose, its metabolism is important for lignocellulosic biomass utilization. Here, we report a six-protein module for d-xylose signaling, uptake and regulation in solvent-producing Clostridium beijerinckii. This module consists of a novel three-component system' (a putative periplasmic ABC transporter substrate-binding protein XylFII and a two-component system LytS/YesN) and an ABC-type d-xylose transporter XylFGH. Interestingly, we demonstrate that, although XylFII harbors a transmembrane domain, it is not involved in d-xylose transport. Instead, XylFII acts as a signal sensor to assist the response of LytS/YesN to extracellular d-xylose, thus enabling LytS/YesN to directly activate the transcription of the adjacent xylFGH genes and thereby promote the uptake of d-xylose. To our knowledge, XylFII is a novel single transmembrane sensor that assists two-component system to respond to extracellular sugar molecules. Also of significance, this three-component system' is widely distributed in Firmicutes, indicating that it may play a broad role in this bacterial phylum. The results reported here provide new insights into the regulatory mechanism of d-xylose sensing and transport in bacteria.
英文摘要: d-Xylose is the most abundant fermentable pentose in nature and can serve as a carbon source for many bacterial species. Since d-xylose constitutes the major component of hemicellulose, its metabolism is important for lignocellulosic biomass utilization. Here, we report a six-protein module for d-xylose signaling, uptake and regulation in solvent-producing Clostridium beijerinckii. This module consists of a novel three-component system' (a putative periplasmic ABC transporter substrate-binding protein XylFII and a two-component system LytS/YesN) and an ABC-type d-xylose transporter XylFGH. Interestingly, we demonstrate that, although XylFII harbors a transmembrane domain, it is not involved in d-xylose transport. Instead, XylFII acts as a signal sensor to assist the response of LytS/YesN to extracellular d-xylose, thus enabling LytS/YesN to directly activate the transcription of the adjacent xylFGH genes and thereby promote the uptake of d-xylose. To our knowledge, XylFII is a novel single transmembrane sensor that assists two-component system to respond to extracellular sugar molecules. Also of significance, this three-component system' is widely distributed in Firmicutes, indicating that it may play a broad role in this bacterial phylum. The results reported here provide new insights into the regulatory mechanism of d-xylose sensing and transport in bacteria.
刊物名称: MOLECULAR MICROBIOLOGY
英文刊物名称: MOLECULAR MICROBIOLOGY
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学科: Biochemistry & Molecular Biology; Microbiology
英文学科: Biochemistry & Molecular Biology; Microbiology
影响因子: 4.419
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论文类别: Article
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