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论文题目: The Crc protein participates in down-regulation of the Lon gene to promote rhamnolipid production and rhl quorum sensing in Pseudomonas aeruginosa
英文论文题目: The Crc protein participates in down-regulation of the Lon gene to promote rhamnolipid production and rhl quorum sensing in Pseudomonas aeruginosa
第一作者: Yang, NN; Ding, ST; Chen, FF; Zhang, X; Xia, YJ; Di, HX; Cao, Q; Deng, X; Wu, M; Wong, CCL; Tian, XX; Yang, CG; Zhao, J; Lan, LF
英文第一作者: Yang, NN; Ding, ST; Chen, FF; Zhang, X; Xia, YJ; Di, HX; Cao, Q; Deng, X; Wu, M; Wong, CCL; Tian, XX; Yang, CG; Zhao, J; Lan, LF
联系作者: Lan, LF (reprint author), Chinese Acad Sci, Shanghai Inst Mat Med, 555 Zuchongzhi Rd,Pudong Zhangjiang Hitech Pk, Shanghai 201203, Peoples R China.
英文联系作者: Lan, LF (reprint author), Chinese Acad Sci, Shanghai Inst Mat Med, 555 Zuchongzhi Rd,Pudong Zhangjiang Hitech Pk, Shanghai 201203, Peoples R China.
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发表年度: 2015
卷: 96
期: 3
页码: 526-547
摘要: Rhamnolipid acts as a virulence factor during Pseudomonas aeruginosa infection. Here, we show that deletion of the catabolite repression control (crc) gene in P.aeruginosa leads to a rhamnolipid-negative phenotype. This effect is mediated by the down-regulation of rhl quorum sensing (QS). We discover that a disruption of the gene encoding the Lon protease entirely offsets the effect of crc deletion on the production of both rhamnolipid and rhlQS signal C4-HSL. Crc is unable to bind lon mRNAin vitro in the absence of the RNA chaperon Hfq, while Crc contributes to Hfq-mediated repression of the lon gene expression at a posttranscriptional level. Deletion of crc, which results in up-regulation of lon, significantly reduces the in vivo stability and abundance of the RhlI protein that synthesizes C4-HSL, causing the attenuation of rhlQS. Lon is also capable of degrading the RhlI protein in vitro. In addition, constitutive expression of rhlI suppresses the defects of the crc deletion mutant in rhamnolipid, C4-HSL and virulence on lettuce leaves. This study therefore uncovers a novel posttranscriptional regulatory cascade, Crc-Hfq/Lon/RhlI, for the regulation of rhamnolipid production and rhlQS in P.aeruginosa.
英文摘要: Rhamnolipid acts as a virulence factor during Pseudomonas aeruginosa infection. Here, we show that deletion of the catabolite repression control (crc) gene in P.aeruginosa leads to a rhamnolipid-negative phenotype. This effect is mediated by the down-regulation of rhl quorum sensing (QS). We discover that a disruption of the gene encoding the Lon protease entirely offsets the effect of crc deletion on the production of both rhamnolipid and rhlQS signal C4-HSL. Crc is unable to bind lon mRNAin vitro in the absence of the RNA chaperon Hfq, while Crc contributes to Hfq-mediated repression of the lon gene expression at a posttranscriptional level. Deletion of crc, which results in up-regulation of lon, significantly reduces the in vivo stability and abundance of the RhlI protein that synthesizes C4-HSL, causing the attenuation of rhlQS. Lon is also capable of degrading the RhlI protein in vitro. In addition, constitutive expression of rhlI suppresses the defects of the crc deletion mutant in rhamnolipid, C4-HSL and virulence on lettuce leaves. This study therefore uncovers a novel posttranscriptional regulatory cascade, Crc-Hfq/Lon/RhlI, for the regulation of rhamnolipid production and rhlQS in P.aeruginosa.
刊物名称: MOLECULAR MICROBIOLOGY
英文刊物名称: MOLECULAR MICROBIOLOGY
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学科: Biochemistry & Molecular Biology; Microbiology
英文学科: Biochemistry & Molecular Biology; Microbiology
影响因子: 4.419
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论文类别: Article
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