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论文题目: Generation and Characterization of Transgenic Mice Expressing Mouse Ins1 Promoter for Pancreatic beta-Cell-Specific Gene Overexpression and Knockout
英文论文题目: Generation and Characterization of Transgenic Mice Expressing Mouse Ins1 Promoter for Pancreatic beta-Cell-Specific Gene Overexpression and Knockout
第一作者: Cheng, YL; Su, YT; Shan, AJ; Jiang, XL; Ma, QY; Wang, WQ; Ning, G; Cao, YA
英文第一作者: Cheng, YL; Su, YT; Shan, AJ; Jiang, XL; Ma, QY; Wang, WQ; Ning, G; Cao, YA
联系作者: Ning, G (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Lab Endocrinol & Metab, Beijing 100864, Peoples R China.
英文联系作者: Ning, G (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Lab Endocrinol & Metab, Beijing 100864, Peoples R China.
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发表年度: 2015
卷: 156
期: 7
页码: 2724-2731
摘要: The technologies for pancreatic beta-cell-specific gene overexpression or knockout are fundamental for investigations of functional genes in vivo. Here we generated the Ins1-Cre-Dsred and Ins1-rtTA mouse models, which expressed the Cre recombinase or reverse tetracycline regulatable transactivator (rtTA) without hGH minigene under the control of mouse Ins1 promoter. Our data showed that the Cre-mediated recombination and rtTA-mediated activation could be efficiently detected at embryonic day 13.5 when these models were crossed with the reporter mice (ROSA(mT/mG) or tetO-HIST1H2BJ/GFP). The Cre and rtTA expression was restricted to beta-cells without leakage in the brain and other tissues. Moreover, both the transgenic lines showed normal glucose tolerance and insulin secretion. These results suggested that the Ins1-Cre-Dsred and Ins1-rtTA mice could be used to knock out or overexpress target genes in embryos and adults to facilitate beta-cell researches.
英文摘要: The technologies for pancreatic beta-cell-specific gene overexpression or knockout are fundamental for investigations of functional genes in vivo. Here we generated the Ins1-Cre-Dsred and Ins1-rtTA mouse models, which expressed the Cre recombinase or reverse tetracycline regulatable transactivator (rtTA) without hGH minigene under the control of mouse Ins1 promoter. Our data showed that the Cre-mediated recombination and rtTA-mediated activation could be efficiently detected at embryonic day 13.5 when these models were crossed with the reporter mice (ROSA(mT/mG) or tetO-HIST1H2BJ/GFP). The Cre and rtTA expression was restricted to beta-cells without leakage in the brain and other tissues. Moreover, both the transgenic lines showed normal glucose tolerance and insulin secretion. These results suggested that the Ins1-Cre-Dsred and Ins1-rtTA mice could be used to knock out or overexpress target genes in embryos and adults to facilitate beta-cell researches.
刊物名称: ENDOCRINOLOGY
英文刊物名称: ENDOCRINOLOGY
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学科: Endocrinology & Metabolism
英文学科: Endocrinology & Metabolism
影响因子: 4.503
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论文类别: Article
英文论文类别: Article
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