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论文题目: Exome sequencing reveals mutant genes with low penetrance involved in MEN2A-associated tumorigenesis
英文论文题目: Exome sequencing reveals mutant genes with low penetrance involved in MEN2A-associated tumorigenesis
第一作者: Cai, J; Li, L; Ye, L; Jiang, XH; Shen, LY; Gao, ZB; Fang, WY; Huang, FJ; Su, TW; Zhou, YL; Wang, WQ; Ning, G
英文第一作者: Cai, J; Li, L; Ye, L; Jiang, XH; Shen, LY; Gao, ZB; Fang, WY; Huang, FJ; Su, TW; Zhou, YL; Wang, WQ; Ning, G
联系作者: Ning, G (reprint author), Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Shanghai Clin Ctr Endocrine & Metab Dis, 197 Ruijin 2nd Rd, Shanghai 200025, Peoples R China.
英文联系作者: Ning, G (reprint author), Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Shanghai Clin Ctr Endocrine & Metab Dis, 197 Ruijin 2nd Rd, Shanghai 200025, Peoples R China.
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发表年度: 2015
卷: 22
期: 1
页码: 23-33
摘要: Activating rearranged during transfection (RET) mutations function as the initiating causative mutation for multiple endocrine neoplasia type 2A (MEN2A). However, no conclusive findings regarding the non-RET genetic events have been reported. This is the first study, to our knowledge, examining genomic alterations in matched MEN2A-associated tumors. We performed exome sequencing and SNP array analysis of matched MEN2A tumors and germline DNA. Somatic alterations were validated in an independent set of patients using Sanger sequencing. Genes of functional interest were further evaluated. The germline RET mutation was found in all MEN2A-component tumors. Thirty-two somatic mutations were identified in the nine MEN2A-associated tumors, of which 28 (87.5%) were point mutations and 4 (12.5%) were small insertions, duplications, or deletions. We sequenced all the mutations as well as coding sequence regions of the 12 genes in an independent sample set including 35 medullary thyroid cancers (20 MEN2A) and 34 PCCs (22 MEN2A), but found no recurrent mutations. Recurrent alterations were found in 13 genes with either mutations or alterations in copy number, including an EIF4G1 mutation (p. E1147V). Mutation of EIF4G1 led to increased cell proliferation and RET/MAPK phosphorylation, while knockdown of EIF4G1 led to reduced cell proliferation and RET/MAPK phosphorylation in TT, MZ-CRC1, and PC-12 cells. We found fewer somatic mutations in endocrine tumors compared with non-endocrine tumors. RET was the primary driver in MEN2A-associated tumors. However, low-frequency alterations such as EIF4G1 might participate in MEN2A-associated tumorigenesis, possibly by regulating the activity of the RET pathway.
英文摘要: Activating rearranged during transfection (RET) mutations function as the initiating causative mutation for multiple endocrine neoplasia type 2A (MEN2A). However, no conclusive findings regarding the non-RET genetic events have been reported. This is the first study, to our knowledge, examining genomic alterations in matched MEN2A-associated tumors. We performed exome sequencing and SNP array analysis of matched MEN2A tumors and germline DNA. Somatic alterations were validated in an independent set of patients using Sanger sequencing. Genes of functional interest were further evaluated. The germline RET mutation was found in all MEN2A-component tumors. Thirty-two somatic mutations were identified in the nine MEN2A-associated tumors, of which 28 (87.5%) were point mutations and 4 (12.5%) were small insertions, duplications, or deletions. We sequenced all the mutations as well as coding sequence regions of the 12 genes in an independent sample set including 35 medullary thyroid cancers (20 MEN2A) and 34 PCCs (22 MEN2A), but found no recurrent mutations. Recurrent alterations were found in 13 genes with either mutations or alterations in copy number, including an EIF4G1 mutation (p. E1147V). Mutation of EIF4G1 led to increased cell proliferation and RET/MAPK phosphorylation, while knockdown of EIF4G1 led to reduced cell proliferation and RET/MAPK phosphorylation in TT, MZ-CRC1, and PC-12 cells. We found fewer somatic mutations in endocrine tumors compared with non-endocrine tumors. RET was the primary driver in MEN2A-associated tumors. However, low-frequency alterations such as EIF4G1 might participate in MEN2A-associated tumorigenesis, possibly by regulating the activity of the RET pathway.
刊物名称: ENDOCRINE-RELATED CANCER
英文刊物名称: ENDOCRINE-RELATED CANCER
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学科: Oncology; Endocrinology & Metabolism
英文学科: Oncology; Endocrinology & Metabolism
影响因子: 4.805
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论文类别: Article
英文论文类别: Article
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