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论文题目: Structure of a yeast spliceosome at 3.6-angstrom resolution
英文论文题目: Structure of a yeast spliceosome at 3.6-angstrom resolution
第一作者: Yan, CY; Hang, J; Wan, RX; Huang, M; Wong, CCL; Shi, YG
英文第一作者: Yan, CY; Hang, J; Wan, RX; Huang, M; Wong, CCL; Shi, YG
联系作者: Shi, YG (reprint author), Tsinghua Univ, Minist Educ, Key Lab Prot Sci,Sch Life Sci, Tsinghua Peking Joint Ctr Life Sci,Ctr Struct Bio, Beijing 100084, Peoples R China.
英文联系作者: Shi, YG (reprint author), Tsinghua Univ, Minist Educ, Key Lab Prot Sci,Sch Life Sci, Tsinghua Peking Joint Ctr Life Sci,Ctr Struct Bio, Beijing 100084, Peoples R China.
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发表年度: 2015
卷: 349
期: 6253
页码: 1182-1191
摘要: Splicing of precursor messenger RNA (pre-mRNA) in yeast is executed by the spliceosome, which consists of five small nuclear ribonucleoproteins (snRNPs), NTC (nineteen complex), NTC-related proteins (NTR), and a number of associated enzymes and cofactors. Here, we report the three-dimensional structure of a Schizosaccharomyces pombe spliceosome at 3.6-angstrom resolution, revealed by means of single-particle cryogenic electron microscopy. This spliceosome contains U2 and U5 snRNPs, NTC, NTR, U6 small nuclear RNA, and an RNA intron lariat. The atomic model includes 10,574 amino acids from 37 proteins and four RNA molecules, with a combined molecular mass of approximately 1.3 megadaltons. Spp42 (Prp8 in Saccharomyces cerevisiae), the key protein component of the U5 snRNP, forms a central scaffold and anchors the catalytic center. Both the morphology and the placement of protein components appear to have evolved to facilitate the dynamic process of pre-mRNA splicing. Our near-atomic-resolution structure of a central spliceosome provides a molecular framework for mechanistic understanding of pre-mRNA splicing.
英文摘要: Splicing of precursor messenger RNA (pre-mRNA) in yeast is executed by the spliceosome, which consists of five small nuclear ribonucleoproteins (snRNPs), NTC (nineteen complex), NTC-related proteins (NTR), and a number of associated enzymes and cofactors. Here, we report the three-dimensional structure of a Schizosaccharomyces pombe spliceosome at 3.6-angstrom resolution, revealed by means of single-particle cryogenic electron microscopy. This spliceosome contains U2 and U5 snRNPs, NTC, NTR, U6 small nuclear RNA, and an RNA intron lariat. The atomic model includes 10,574 amino acids from 37 proteins and four RNA molecules, with a combined molecular mass of approximately 1.3 megadaltons. Spp42 (Prp8 in Saccharomyces cerevisiae), the key protein component of the U5 snRNP, forms a central scaffold and anchors the catalytic center. Both the morphology and the placement of protein components appear to have evolved to facilitate the dynamic process of pre-mRNA splicing. Our near-atomic-resolution structure of a central spliceosome provides a molecular framework for mechanistic understanding of pre-mRNA splicing.
刊物名称: SCIENCE
英文刊物名称: SCIENCE
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学科: Science & Technology - Other Topics
英文学科: Science & Technology - Other Topics
影响因子: 33.611
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论文类别: Article
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