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论文题目: Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling
英文论文题目: Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling
第一作者: Zhang, F; Yao, J; Ke, JY; Zhang, L; Lam, VQ; Xin, XF; Zhou, XE; Chen, J; Brunzelle, J; Griffin, PR; Zhou, MG; Xu, HE; Melcher, K; He, SY
英文第一作者: Zhang, F; Yao, J; Ke, JY; Zhang, L; Lam, VQ; Xin, XF; Zhou, XE; Chen, J; Brunzelle, J; Griffin, PR; Zhou, MG; Xu, HE; Melcher, K; He, SY
联系作者: Xu, HE (reprint author), Van Andel Res Inst, Lab Struct Sci, Grand Rapids, MI 49503 USA.
英文联系作者: Xu, HE (reprint author), Van Andel Res Inst, Lab Struct Sci, Grand Rapids, MI 49503 USA.
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发表年度: 2015
卷: 525
期: 7568
页码: 269-+
摘要: The plant hormone jasmonate plays crucial roles in regulating plant responses to herbivorous insects and microbial pathogens and is an important regulator of plant growth and development(1-7). Keymediators of jasmonate signalling include MYC transcription factors, which are repressed by jasmonate ZIM-domain (JAZ) transcriptional repressors in the resting state. In the presence of active jasmonate, JAZ proteins function as jasmonate co-receptors by forming a hormone-dependent complex with COI1, the F-box subunit of an SCF-type ubiquitin E3 ligase(8-11). The hormone-dependent formation of the COI1-JAZ co-receptor complex leads to ubiquitination and proteasome-dependent degradation of JAZ repressors and release of MYC proteins from transcriptional repression(3,10,12). The mechanism by which JAZ proteins repress MYC transcription factors and how JAZ proteins switch between the repressor function in the absence of hormone and the co-receptor function in the presence of hormone remain enigmatic. Here we show that Arabidopsis MYC3 undergoes pronounced conformational changes when bound to the conserved Jas motif of the JAZ9 repressor. The Jas motif, previously shown to bind to hormone as a partly unwound helix, forms a complete alpha-helix that displaces the amino (N)-terminal helix ofMYC3 and becomes an integral part of theMYC N-terminal fold. In this position, the Jas helix competitively inhibits MYC3 interaction with the MED25 subunit of the transcriptional Mediator complex. Our structural and functional studies elucidate a dynamic molecular switchmechanism that governs the repression and activation of a major plant hormone pathway.
英文摘要: The plant hormone jasmonate plays crucial roles in regulating plant responses to herbivorous insects and microbial pathogens and is an important regulator of plant growth and development(1-7). Keymediators of jasmonate signalling include MYC transcription factors, which are repressed by jasmonate ZIM-domain (JAZ) transcriptional repressors in the resting state. In the presence of active jasmonate, JAZ proteins function as jasmonate co-receptors by forming a hormone-dependent complex with COI1, the F-box subunit of an SCF-type ubiquitin E3 ligase(8-11). The hormone-dependent formation of the COI1-JAZ co-receptor complex leads to ubiquitination and proteasome-dependent degradation of JAZ repressors and release of MYC proteins from transcriptional repression(3,10,12). The mechanism by which JAZ proteins repress MYC transcription factors and how JAZ proteins switch between the repressor function in the absence of hormone and the co-receptor function in the presence of hormone remain enigmatic. Here we show that Arabidopsis MYC3 undergoes pronounced conformational changes when bound to the conserved Jas motif of the JAZ9 repressor. The Jas motif, previously shown to bind to hormone as a partly unwound helix, forms a complete alpha-helix that displaces the amino (N)-terminal helix ofMYC3 and becomes an integral part of theMYC N-terminal fold. In this position, the Jas helix competitively inhibits MYC3 interaction with the MED25 subunit of the transcriptional Mediator complex. Our structural and functional studies elucidate a dynamic molecular switchmechanism that governs the repression and activation of a major plant hormone pathway.
刊物名称: NATURE
英文刊物名称: NATURE
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学科: Science & Technology - Other Topics
英文学科: Science & Technology - Other Topics
影响因子: 41.456
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论文类别: Article
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