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论文题目: Systematic network assessment of the carcinogenic activities of cadmium
英文论文题目: Systematic network assessment of the carcinogenic activities of cadmium
第一作者: Chen, PZ; Duan, XH; Li, M; Huang, C; Li, JQ; Chu, RA; Ying, H; Song, HY; Jia, XD; Ba, Q; Wang, H
英文第一作者: Chen, PZ; Duan, XH; Li, M; Huang, C; Li, JQ; Chu, RA; Ying, H; Song, HY; Jia, XD; Ba, Q; Wang, H
联系作者: Ba, Q; Wang, H (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Rm 2208,New Life Bldg A,320 Yueyang Rd, Shanghai 200031, Peoples R China.
英文联系作者: Ba, Q; Wang, H (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Rm 2208,New Life Bldg A,320 Yueyang Rd, Shanghai 200031, Peoples R China.
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发表年度: 2016
卷: 310
期:
页码: 150-158
摘要: Cadmium has been defined as type I carcinogen for humans, but the underlying mechanisms of its carcinogenic activity and its influence on protein-protein interactions in cells are not fully elucidated. The aim of the current study was to evaluate, systematically, the carcinogenic activity of cadmium with systems biology approaches. From a literature search of 209 studies that performed with cellular models, 208 proteins influenced by cadmium exposure were identified. All of these were assessed by Western blotting and were recognized as key nodes in network analyses. The protein-protein functional interaction networks were constructed with NetBox software and visualized with Cytoscape software. These cadmium-rewired genes were used to construct a scale-free, highly connected biological protein interaction network with 850 nodes and 8770 edges. Of the network, nine key modules were identified and 60 key signaling pathways, including the estrogen, RAS, PI3K-Akt, NF-kappa B, HIF-1 alpha, Jak-STAT, and TGF-beta signaling pathways, were significantly enriched. With breast cancer, colorectal and prostate cancer cellular models, we validated the key node genes in the network that had been previously reported or inferred form the network by Western blotting methods, including STAT3, JNK, p38, SMAD2/3, P65, AKTI, and HIF-1 alpha. These results suggested the established network was robust and provided a systematic view of the carcinogenic activities of cadmium in human.
英文摘要: Cadmium has been defined as type I carcinogen for humans, but the underlying mechanisms of its carcinogenic activity and its influence on protein-protein interactions in cells are not fully elucidated. The aim of the current study was to evaluate, systematically, the carcinogenic activity of cadmium with systems biology approaches. From a literature search of 209 studies that performed with cellular models, 208 proteins influenced by cadmium exposure were identified. All of these were assessed by Western blotting and were recognized as key nodes in network analyses. The protein-protein functional interaction networks were constructed with NetBox software and visualized with Cytoscape software. These cadmium-rewired genes were used to construct a scale-free, highly connected biological protein interaction network with 850 nodes and 8770 edges. Of the network, nine key modules were identified and 60 key signaling pathways, including the estrogen, RAS, PI3K-Akt, NF-kappa B, HIF-1 alpha, Jak-STAT, and TGF-beta signaling pathways, were significantly enriched. With breast cancer, colorectal and prostate cancer cellular models, we validated the key node genes in the network that had been previously reported or inferred form the network by Western blotting methods, including STAT3, JNK, p38, SMAD2/3, P65, AKTI, and HIF-1 alpha. These results suggested the established network was robust and provided a systematic view of the carcinogenic activities of cadmium in human.
刊物名称: TOXICOLOGY AND APPLIED PHARMACOLOGY
英文刊物名称: TOXICOLOGY AND APPLIED PHARMACOLOGY
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学科: Pharmacology & Pharmacy; Toxicology
英文学科: Pharmacology & Pharmacy; Toxicology
影响因子: 3.791
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论文类别: Article
英文论文类别: Article
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