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论文题目: Filamentation of Metabolic Enzymes in Saccharomyces cerevisiae
英文论文题目: Filamentation of Metabolic Enzymes in Saccharomyces cerevisiae
第一作者: Shen, QJ; Kassim, H; Huang, Y; Li, H; Zhang, J; Li, G; Wang, PY; Yan, J; Ye, FF; Liu, JL
英文第一作者: Shen, QJ; Kassim, H; Huang, Y; Li, H; Zhang, J; Li, G; Wang, PY; Yan, J; Ye, FF; Liu, JL
联系作者: Liu, JL (reprint author), Univ Oxford, Dept Physiol Anat & Genet, Funct Genom Unit, MRC, Oxford OX1 3PT, England.
英文联系作者: Liu, JL (reprint author), Univ Oxford, Dept Physiol Anat & Genet, Funct Genom Unit, MRC, Oxford OX1 3PT, England.
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发表年度: 2016
卷: 43
期: 6
页码: 393-404
摘要: Compartmentation via filamentation has recently emerged as a novel mechanism for metabolic regulation. In order to identify filament forming metabolic enzymes systematically, we performed a genome-wide screening of all strains available from an open reading frame GFP collection in Saccharomyces cerevisiae. We discovered nine novel filament-forming proteins and also confirmed those identified previously. From the 4159 strains, we found 23 proteins, mostly metabolic enzymes, which are capable of forming filaments in vivo. In silico protein-protein interaction analysis suggests that these filament-forming proteins can be clustered into several groups, including translational initiation machinery and glucose and nitrogen metabolic pathways. Using glutamine-utilising enzymes as examples, we found that the culture conditions affect the occurrence and length of the metabolic filaments. Furthermore, we found that two CTP synthases (Ura7p and Ura8p) and two asparagine synthetases (Asn1p and Asn2p) form filaments both in the cytoplasm and in the nucleus. Live imaging analyses suggest that metabolic filaments undergo sub-diffusion. Taken together, our genome-wide screening identifies additional filament-forming proteins in S. cerevisiae and suggests that filamentation of metabolic enzymes is more general than currently appreciated.
英文摘要: Compartmentation via filamentation has recently emerged as a novel mechanism for metabolic regulation. In order to identify filament forming metabolic enzymes systematically, we performed a genome-wide screening of all strains available from an open reading frame GFP collection in Saccharomyces cerevisiae. We discovered nine novel filament-forming proteins and also confirmed those identified previously. From the 4159 strains, we found 23 proteins, mostly metabolic enzymes, which are capable of forming filaments in vivo. In silico protein-protein interaction analysis suggests that these filament-forming proteins can be clustered into several groups, including translational initiation machinery and glucose and nitrogen metabolic pathways. Using glutamine-utilising enzymes as examples, we found that the culture conditions affect the occurrence and length of the metabolic filaments. Furthermore, we found that two CTP synthases (Ura7p and Ura8p) and two asparagine synthetases (Asn1p and Asn2p) form filaments both in the cytoplasm and in the nucleus. Live imaging analyses suggest that metabolic filaments undergo sub-diffusion. Taken together, our genome-wide screening identifies additional filament-forming proteins in S. cerevisiae and suggests that filamentation of metabolic enzymes is more general than currently appreciated.
刊物名称: JOURNAL OF GENETICS AND GENOMICS
英文刊物名称: JOURNAL OF GENETICS AND GENOMICS
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学科: Biochemistry & Molecular Biology; Genetics & Heredity
英文学科: Biochemistry & Molecular Biology; Genetics & Heredity
影响因子: 4.051
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论文类别: Article
英文论文类别: Article
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