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论文题目: Hyperlipidemia-associated gene variations and expression patterns revealed by whole-genome and transcriptome sequencing of rabbit models
英文论文题目: Hyperlipidemia-associated gene variations and expression patterns revealed by whole-genome and transcriptome sequencing of rabbit models
第一作者: Wang, Z; Zhang, JF; Li, H; Li, JY; Niimi, M; Ding, GH; Chen, HF; Xu, J; Zhang, HJ; Xu, Z; Dai, YL; Gui, TT; Li, SD; Liu, Z; Wu, SJ; Cao, MS; Zhou, L; Lu, XY; Wang, JX; Yang, J; Fu, YH; Yang, DS; Song, J; Zhu, TQ; Li, S; Ning, B; Wang, ZY; Koike, T; Shiomi, M; Liu, EQ; Chen, LN; Fan, JL; Chen, YE; Li, YX
英文第一作者: Wang, Z; Zhang, JF; Li, H; Li, JY; Niimi, M; Ding, GH; Chen, HF; Xu, J; Zhang, HJ; Xu, Z; Dai, YL; Gui, TT; Li, SD; Liu, Z; Wu, SJ; Cao, MS; Zhou, L; Lu, XY; Wang, JX; Yang, J; Fu, YH; Yang, DS; Song, J; Zhu, TQ; Li, S; Ning, B; Wang, ZY; Koike, T; Shiomi, M; Liu, EQ; Chen, LN; Fan, JL; Chen, YE; Li, YX
联系作者: Li, YX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, Key Lab Computat Biol, Shanghai, Peoples R China.
英文联系作者: Li, YX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, Key Lab Computat Biol, Shanghai, Peoples R China.
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发表年度: 2016
卷: 6
期:
页码: 26942
摘要: The rabbit (Oryctolagus cuniculus) is an important experimental animal for studying human diseases, such as hypercholesterolemia and atherosclerosis. Despite this, genetic information and RNA expression profiling of laboratory rabbits are lacking. Here, we characterized the whole-genome variants of three breeds of the most popular experimental rabbits, New Zealand White (NZW), Japanese White (JW) and Watanabe heritable hyperlipidemic (WHHL) rabbits. Although the genetic diversity of WHHL rabbits was relatively low, they accumulated a large proportion of high-frequency deleterious mutations due to the small population size. Some of the deleterious mutations were associated with the pathophysiology of WHHL rabbits in addition to the LDLR deficiency. Furthermore, we conducted transcriptome sequencing of different organs of both WHHL and cholesterol-rich diet (Chol)-fed NZW rabbits. We found that gene expression profiles of the two rabbit models were essentially similar in the aorta, even though they exhibited different types of hypercholesterolemia. In contrast, Chol-fed rabbits, but not WHHL rabbits, exhibited pronounced inflammatory responses and abnormal lipid metabolism in the liver. These results provide valuable insights into identifying therapeutic targets of hypercholesterolemia and atherosclerosis with rabbit models.
英文摘要: The rabbit (Oryctolagus cuniculus) is an important experimental animal for studying human diseases, such as hypercholesterolemia and atherosclerosis. Despite this, genetic information and RNA expression profiling of laboratory rabbits are lacking. Here, we characterized the whole-genome variants of three breeds of the most popular experimental rabbits, New Zealand White (NZW), Japanese White (JW) and Watanabe heritable hyperlipidemic (WHHL) rabbits. Although the genetic diversity of WHHL rabbits was relatively low, they accumulated a large proportion of high-frequency deleterious mutations due to the small population size. Some of the deleterious mutations were associated with the pathophysiology of WHHL rabbits in addition to the LDLR deficiency. Furthermore, we conducted transcriptome sequencing of different organs of both WHHL and cholesterol-rich diet (Chol)-fed NZW rabbits. We found that gene expression profiles of the two rabbit models were essentially similar in the aorta, even though they exhibited different types of hypercholesterolemia. In contrast, Chol-fed rabbits, but not WHHL rabbits, exhibited pronounced inflammatory responses and abnormal lipid metabolism in the liver. These results provide valuable insights into identifying therapeutic targets of hypercholesterolemia and atherosclerosis with rabbit models.
刊物名称: SCIENTIFIC REPORTS
英文刊物名称: SCIENTIFIC REPORTS
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学科: Multidisciplinary Sciences
英文学科: Multidisciplinary Sciences
影响因子: 4.259
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论文类别: Article
英文论文类别: Article
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