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论文题目: Hepatocellular carcinoma cell lines retain the genomic and transcriptomic landscapes of primary human cancers
英文论文题目: Hepatocellular carcinoma cell lines retain the genomic and transcriptomic landscapes of primary human cancers
第一作者: Qiu, ZX; Zou, KK; Zhuang, LP; Qin, JJ; Li, H; Li, C; Zhang, ZT; Chen, XT; Cen, J; Meng, ZQ; Zhang, HB; Li, YX; Hui, LJ
英文第一作者: Qiu, ZX; Zou, KK; Zhuang, LP; Qin, JJ; Li, H; Li, C; Zhang, ZT; Chen, XT; Cen, J; Meng, ZQ; Zhang, HB; Li, YX; Hui, LJ
联系作者: Hui, LJ (reprint author), Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.
英文联系作者: Hui, LJ (reprint author), Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.
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发表年度: 2016
卷: 6
期:
页码: 27411
摘要: Hepatocellular carcinoma (HCC) cell lines are useful in vitro models for the study of primary HCCs. Because cell lines acquire additional mutations in culture, it is important to understand to what extent HCC cell lines retain the genetic landscapes of primary HCCs. Most HCC cell lines were established during the last century, precluding comparison between cell lines and primary cancers. In this study, 9 Chinese HCC cell lines with matched patient-derived cells at low passages (PDCs) were established in the defined culture condition. Whole genome analyses of 4 HCC cell lines showed that genomic mutation landscapes, including mutations, copy number alterations (CNAs) and HBV integrations, were highly stable during cell line establishment. Importantly, genetic alterations in cancer drivers and druggable genes were reserved in cell lines. HCC cell lines also retained gene expression patterns of primary HCCs during in vitro culture. Finally, sequential analysis of HCC cell lines and PDCs at different passages revealed their comparable and stable genomic and transcriptomic levels if maintained within proper passages. These results show that HCC cell lines largely retain the genomic and transcriptomic landscapes of primary HCCs, thus laying the rationale for testing HCC cell lines as preclinical models in precision medicine.
英文摘要: Hepatocellular carcinoma (HCC) cell lines are useful in vitro models for the study of primary HCCs. Because cell lines acquire additional mutations in culture, it is important to understand to what extent HCC cell lines retain the genetic landscapes of primary HCCs. Most HCC cell lines were established during the last century, precluding comparison between cell lines and primary cancers. In this study, 9 Chinese HCC cell lines with matched patient-derived cells at low passages (PDCs) were established in the defined culture condition. Whole genome analyses of 4 HCC cell lines showed that genomic mutation landscapes, including mutations, copy number alterations (CNAs) and HBV integrations, were highly stable during cell line establishment. Importantly, genetic alterations in cancer drivers and druggable genes were reserved in cell lines. HCC cell lines also retained gene expression patterns of primary HCCs during in vitro culture. Finally, sequential analysis of HCC cell lines and PDCs at different passages revealed their comparable and stable genomic and transcriptomic levels if maintained within proper passages. These results show that HCC cell lines largely retain the genomic and transcriptomic landscapes of primary HCCs, thus laying the rationale for testing HCC cell lines as preclinical models in precision medicine.
刊物名称: SCIENTIFIC REPORTS
英文刊物名称: SCIENTIFIC REPORTS
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学科: Multidisciplinary Sciences
英文学科: Multidisciplinary Sciences
影响因子: 4.259
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论文类别: Article
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