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论文题目: Peripheral Blood MicroRNA Expression Profiles in Alzheimer's Disease: Screening, Validation, Association with Clinical Phenotype and Implications for Molecular Mechanism
英文论文题目: Peripheral Blood MicroRNA Expression Profiles in Alzheimer's Disease: Screening, Validation, Association with Clinical Phenotype and Implications for Molecular Mechanism
第一作者: Ren, RJ; Zhang, YF; Dammer, EB; Zhou, Y; Wang, LL; Liu, XH; Feng, BL; Jiang, GX; Chen, SD; Wang, G; Cheng, Q
英文第一作者: Ren, RJ; Zhang, YF; Dammer, EB; Zhou, Y; Wang, LL; Liu, XH; Feng, BL; Jiang, GX; Chen, SD; Wang, G; Cheng, Q
联系作者: Chen, SD; Wang, G; Cheng, Q (reprint author), Shanghai Jiao Tong Univ, Dept Neurol, Sch Med, Ruijin Hosp, Shanghai 200025, Peoples R China.
英文联系作者: Chen, SD; Wang, G; Cheng, Q (reprint author), Shanghai Jiao Tong Univ, Dept Neurol, Sch Med, Ruijin Hosp, Shanghai 200025, Peoples R China.
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发表年度: 2016
卷: 53
期: 8
页码: 5772-5781
摘要: A series of investigations have been performed regarding microRNA (miRNA, miR) of Alzheimer's disease (AD) patients. However, most of these used microarray with neither validation by PCR nor any follow-up on the biological mechanism implicated by findings. Further, there were rarely any analyses linking clinical phenotype of de novo, drug-naive patients to cellular pathogenic mechanism(s) to date. Microarray screening followed by validation via quantitative PCR (Q-PCR) assays and the relationship between miRNAs and phenotypic indices were evaluated. Additionally, the cellular mechanism of miRNAs through effects of beta-site amyloid precursor protein (APP) cleaving enzyme (BACE1) was assessed. We identified 2 specific differentially expressed (DE) miRNAs (miR-339 and miR-425) as potential diagnostic biomarkers for AD and revealed that these DE miRNAs could be involved in modulating the pathogenesis of AD via BACE1 protein inhibition. The findings presented here reveal a detailed snapshot of the profile of peripheral blood mononuclear cells (PBMC) miRNA changes in AD patients, association with clinical phenotype, and potential roles in cellular pathogenesis.
英文摘要: A series of investigations have been performed regarding microRNA (miRNA, miR) of Alzheimer's disease (AD) patients. However, most of these used microarray with neither validation by PCR nor any follow-up on the biological mechanism implicated by findings. Further, there were rarely any analyses linking clinical phenotype of de novo, drug-naive patients to cellular pathogenic mechanism(s) to date. Microarray screening followed by validation via quantitative PCR (Q-PCR) assays and the relationship between miRNAs and phenotypic indices were evaluated. Additionally, the cellular mechanism of miRNAs through effects of beta-site amyloid precursor protein (APP) cleaving enzyme (BACE1) was assessed. We identified 2 specific differentially expressed (DE) miRNAs (miR-339 and miR-425) as potential diagnostic biomarkers for AD and revealed that these DE miRNAs could be involved in modulating the pathogenesis of AD via BACE1 protein inhibition. The findings presented here reveal a detailed snapshot of the profile of peripheral blood mononuclear cells (PBMC) miRNA changes in AD patients, association with clinical phenotype, and potential roles in cellular pathogenesis.
刊物名称: MOLECULAR NEUROBIOLOGY
英文刊物名称: MOLECULAR NEUROBIOLOGY
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学科: Neurosciences
英文学科: Neurosciences
影响因子: 6.19
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论文类别: Article
英文论文类别: Article
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