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论文题目: Simultaneous Raising of Rabbit Monoclonal Antibodies to Fluoroquinolones with Diverse Recognition Functionalities via Single Mixture Immunization
英文论文题目: Simultaneous Raising of Rabbit Monoclonal Antibodies to Fluoroquinolones with Diverse Recognition Functionalities via Single Mixture Immunization
第一作者: Liu, N; Zhao, ZY; Tan, YL; Lu, L; Wang, L; Liao, YC; Beloglazova, N; De Saeger, S; Zheng, XD; Wu, AB
英文第一作者: Liu, N; Zhao, ZY; Tan, YL; Lu, L; Wang, L; Liao, YC; Beloglazova, N; De Saeger, S; Zheng, XD; Wu, AB
联系作者: Wu, AB (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Key Lab Food Safety Res, 294 Taiyuan Rd, Shanghai 200031, Peoples R China.
英文联系作者: Wu, AB (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Key Lab Food Safety Res, 294 Taiyuan Rd, Shanghai 200031, Peoples R China.
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发表年度: 2016
卷: 88
期: 2
页码: 1246-1252
摘要: Highly specific monoclonal and polyclonal antibodies are the key components in a diverse set of immunoassay applications, from research work to routine monitoring and analysis. In the current manuscript, combinatorial strategies for a single mixture immunization, screening and rabbit hybridoma cell technology were described. Fluoroquinolones (FQs) drugs were chosen as representative analytes. Six FQs were conjugated with bovine serum albumin and used as immunogens for subsequent immunization, while a mixture of all was injected for coimmunization. The hybridomas obtained against the individual and multiple FQs were used for the production of diverse varieties of rabbit monoclonal antibodies (RabMAbs) against the target analytes. As was proven by indirect competitive ELISA and quantitative lateral flow immunoassay, this approach opens a new way for simultaneously obtaining functional monoclonal antibodies which are capable of recognizing both individual and multiple analytes in a single preparation circle. This addresses various needs of different monitoring regulations as analytical methodology advances.
英文摘要: Highly specific monoclonal and polyclonal antibodies are the key components in a diverse set of immunoassay applications, from research work to routine monitoring and analysis. In the current manuscript, combinatorial strategies for a single mixture immunization, screening and rabbit hybridoma cell technology were described. Fluoroquinolones (FQs) drugs were chosen as representative analytes. Six FQs were conjugated with bovine serum albumin and used as immunogens for subsequent immunization, while a mixture of all was injected for coimmunization. The hybridomas obtained against the individual and multiple FQs were used for the production of diverse varieties of rabbit monoclonal antibodies (RabMAbs) against the target analytes. As was proven by indirect competitive ELISA and quantitative lateral flow immunoassay, this approach opens a new way for simultaneously obtaining functional monoclonal antibodies which are capable of recognizing both individual and multiple analytes in a single preparation circle. This addresses various needs of different monitoring regulations as analytical methodology advances.
刊物名称: ANALYTICAL CHEMISTRY
英文刊物名称: ANALYTICAL CHEMISTRY
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学科: Chemistry, Analytical
英文学科: Chemistry, Analytical
影响因子: 6.32
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论文类别: Article
英文论文类别: Article
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