论文库首页  论文库
 
论文编号:
论文题目: GASZ and mitofusin-mediated mitochondrial functions are crucial for spermatogenesis
英文论文题目: GASZ and mitofusin-mediated mitochondrial functions are crucial for spermatogenesis
第一作者: Zhang, JJ; Wang, Q; Wang, MS; Jiang, MZ; Wang, YS; Sun, Y; Wang, JP; Xie, TR; Tang, C; Tang, NN; Song, HL; Cui, D; Chao, RH; Ding, SZ; Ni, B; Chen, XJ; Wang, Y
英文第一作者: Zhang, JJ; Wang, Q; Wang, MS; Jiang, MZ; Wang, YS; Sun, Y; Wang, JP; Xie, TR; Tang, C; Tang, NN; Song, HL; Cui, D; Chao, RH; Ding, SZ; Ni, B; Chen, XJ; Wang, Y
联系作者: Wang, Y (reprint author), E China Normal Univ, Shanghai Key Lab Regulatory Biol, Inst Biomed Sci, Shanghai 200062, Peoples R China.
英文联系作者: Wang, Y (reprint author), E China Normal Univ, Shanghai Key Lab Regulatory Biol, Inst Biomed Sci, Shanghai 200062, Peoples R China.
外单位作者单位:
英文外单位作者单位:
发表年度: 2016
卷: 17
期: 2
页码: 220-234
摘要: Nuage is an electron-dense cytoplasmic structure in germ cells that contains ribonucleoproteins and participates in piRNA biosynthesis. Despite the observation that clustered mitochondria are associated with a specific type of nuage called intermitochondrial cement (pi-body), the importance of mitochondrial functions in nuage formation and spermatogenesis is yet to be determined. We show that a germ cell-specific protein GASZ contains a functional mitochondrial targeting signal and is largely localized at mitochondria both endogenously in germ cells and in somatic cells when ectopically expressed. In addition, GASZ interacts with itself at the outer membrane of mitochondria and promotes mitofusion in a mitofusin/MFN-dependent manner. In mice, deletion of the mitochondrial targeting signal reveals that mitochondrial localization of GASZ is essential for nuage formation, mitochondrial clustering, transposon repression, and spermatogenesis. MFN1 deficiency also leads to defects in mitochondrial activity and male infertility. Our data thus reveal a requirement for GASZ and MFN-mediated mitofusion during spermatogenesis.
英文摘要: Nuage is an electron-dense cytoplasmic structure in germ cells that contains ribonucleoproteins and participates in piRNA biosynthesis. Despite the observation that clustered mitochondria are associated with a specific type of nuage called intermitochondrial cement (pi-body), the importance of mitochondrial functions in nuage formation and spermatogenesis is yet to be determined. We show that a germ cell-specific protein GASZ contains a functional mitochondrial targeting signal and is largely localized at mitochondria both endogenously in germ cells and in somatic cells when ectopically expressed. In addition, GASZ interacts with itself at the outer membrane of mitochondria and promotes mitofusion in a mitofusin/MFN-dependent manner. In mice, deletion of the mitochondrial targeting signal reveals that mitochondrial localization of GASZ is essential for nuage formation, mitochondrial clustering, transposon repression, and spermatogenesis. MFN1 deficiency also leads to defects in mitochondrial activity and male infertility. Our data thus reveal a requirement for GASZ and MFN-mediated mitofusion during spermatogenesis.
刊物名称: EMBO REPORTS
英文刊物名称: EMBO REPORTS
论文全文:
英文论文全文:
全文链接:
其它备注:
英文其它备注:
学科: Biochemistry & Molecular Biology; Cell Biology
英文学科: Biochemistry & Molecular Biology; Cell Biology
影响因子: 8.568
第一作者所在部门:
英文第一作者所在部门:
论文出处:
英文论文出处:
论文类别: Article
英文论文类别: Article
参与作者:
英文参与作者:
 
2014 中国科学院上海生命科学研究院 版权所有