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论文题目: AKAP95 regulates splicing through scaffolding RNAs and RNA processing factors
英文论文题目: AKAP95 regulates splicing through scaffolding RNAs and RNA processing factors
第一作者: Hu, J; Khodadadi-Jamayran, A; Mao, MW; Shah, K; Yang, ZH; Nasim, MT; Wang, ZF; Jiang, H
英文第一作者: Hu, J; Khodadadi-Jamayran, A; Mao, MW; Shah, K; Yang, ZH; Nasim, MT; Wang, ZF; Jiang, H
联系作者: Jiang, H (reprint author), Univ Alabama Birmingham, Dept Biochem & Mol Genet, UAB Stem Cell Inst, Sch Med, Birmingham, AL 35294 USA.
英文联系作者: Jiang, H (reprint author), Univ Alabama Birmingham, Dept Biochem & Mol Genet, UAB Stem Cell Inst, Sch Med, Birmingham, AL 35294 USA.
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发表年度: 2016
卷: 7
期:
页码: 13347
摘要: Alternative splicing of pre-mRNAs significantly contributes to the complexity of gene expression in higher organisms, but the regulation of the splice site selection remains incompletely understood. We have previously demonstrated that a chromatin-associated protein, AKAP95, has a remarkable activity in enhancing chromatin transcription. In this study, we show that AKAP95 interacts with many factors involved in transcription and RNA processing, including selective groups of hnRNP proteins, through its N-terminal region, and directly regulates pre-mRNA splicing. AKAP95 binds preferentially to proximal intronic regions on pre-mRNAs in human transcriptome, and this binding requires its zinc-finger domains. By selectively coordinating with hnRNP H/F and U proteins, AKAP95 appears to mainly promote the inclusion of many exons in the genome. AKAP95 also directly interacts with itself. Taken together, our results establish AKAP95 as a mostly positive regulator of pre-mRNA splicing and a possible integrator of transcription and splicing regulation.
英文摘要: Alternative splicing of pre-mRNAs significantly contributes to the complexity of gene expression in higher organisms, but the regulation of the splice site selection remains incompletely understood. We have previously demonstrated that a chromatin-associated protein, AKAP95, has a remarkable activity in enhancing chromatin transcription. In this study, we show that AKAP95 interacts with many factors involved in transcription and RNA processing, including selective groups of hnRNP proteins, through its N-terminal region, and directly regulates pre-mRNA splicing. AKAP95 binds preferentially to proximal intronic regions on pre-mRNAs in human transcriptome, and this binding requires its zinc-finger domains. By selectively coordinating with hnRNP H/F and U proteins, AKAP95 appears to mainly promote the inclusion of many exons in the genome. AKAP95 also directly interacts with itself. Taken together, our results establish AKAP95 as a mostly positive regulator of pre-mRNA splicing and a possible integrator of transcription and splicing regulation.
刊物名称: NATURE COMMUNICATIONS
英文刊物名称: NATURE COMMUNICATIONS
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学科: Multidisciplinary Sciences
英文学科: Multidisciplinary Sciences
影响因子: 12.124
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论文类别: Article
英文论文类别: Article
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