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论文题目: Transplantation of betatrophin-expressing adipose-derived mesenchymal stem cells induces -cell proliferation in diabetic mice
英文论文题目: Transplantation of betatrophin-expressing adipose-derived mesenchymal stem cells induces -cell proliferation in diabetic mice
第一作者: Sun, LL; Liu, TJ; Li, LM; Tang, W; Zou, JJ; Chen, XF; Zheng, JY; Jiang, BG; Shi, YQ
英文第一作者: Sun, LL; Liu, TJ; Li, LM; Tang, W; Zou, JJ; Chen, XF; Zheng, JY; Jiang, BG; Shi, YQ
联系作者: Shi, YQ (reprint author), Second Mil Med Univ, Changzheng Hosp, Dept Endocrinol, 415 Rd Fengyang, Shanghai 200003, Peoples R China.
英文联系作者: Shi, YQ (reprint author), Second Mil Med Univ, Changzheng Hosp, Dept Endocrinol, 415 Rd Fengyang, Shanghai 200003, Peoples R China.
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发表年度: 2017
卷: 39
期: 4
页码: 936-948
摘要: Recent progress in regenerative medicine has suggested that mesenchymal stem cell (MSC)-based therapy is a novel potential cure for diabetes. Betatrophin is a newly identified hormone that can increase the production and expansion of insulin-secreting -cells when administered to mice. In this study, we evaluated the effect of betatrophin overexpression by human adipose-derived MSCs (ADMSCs) by in vitro experiments, as well as following their transplantation into a mice with streptozotocin (STZ)-induced diabetes. The overexpression of betatrophin did not affect the ADMSCs in terms of proliferation, differentiation and morphology. However, the co-culture of human islets with ADMSCs overexpressing betatrophin (ADMSCs-BET) induced islet proliferation, -cell specific transcription factor expression, and the islet production of insulin under the stimulation of glucose or KCl and Arg. In addition, ADMSCs-BET enhanced the anti-inflammatory and anti-apoptotic effects of the co-cultured islets compared with ADMSCs cultured alone. In mice with STZ-induced diabetes, the transplantation of ADMSCs-BET ameliorated the hyperglycemia and weight loss associated with STZ-induced diabetes; ADMSCs-BET also significantly enhanced the ratio of -cells per islet compared to the transplantation of ADMSCs alone. Thus, our study demonstrates a novel strategy for inducing -cell regeneration. ADMSCs-BET may replace insulin injections by increasing the number of endogenous insulin-producing cells in patients with diabetes. This combined strategy of ADMSC transplantation and gene therapy may prove to be a useful therapy for the treatment of diabetes.
英文摘要: Recent progress in regenerative medicine has suggested that mesenchymal stem cell (MSC)-based therapy is a novel potential cure for diabetes. Betatrophin is a newly identified hormone that can increase the production and expansion of insulin-secreting -cells when administered to mice. In this study, we evaluated the effect of betatrophin overexpression by human adipose-derived MSCs (ADMSCs) by in vitro experiments, as well as following their transplantation into a mice with streptozotocin (STZ)-induced diabetes. The overexpression of betatrophin did not affect the ADMSCs in terms of proliferation, differentiation and morphology. However, the co-culture of human islets with ADMSCs overexpressing betatrophin (ADMSCs-BET) induced islet proliferation, -cell specific transcription factor expression, and the islet production of insulin under the stimulation of glucose or KCl and Arg. In addition, ADMSCs-BET enhanced the anti-inflammatory and anti-apoptotic effects of the co-cultured islets compared with ADMSCs cultured alone. In mice with STZ-induced diabetes, the transplantation of ADMSCs-BET ameliorated the hyperglycemia and weight loss associated with STZ-induced diabetes; ADMSCs-BET also significantly enhanced the ratio of -cells per islet compared to the transplantation of ADMSCs alone. Thus, our study demonstrates a novel strategy for inducing -cell regeneration. ADMSCs-BET may replace insulin injections by increasing the number of endogenous insulin-producing cells in patients with diabetes. This combined strategy of ADMSC transplantation and gene therapy may prove to be a useful therapy for the treatment of diabetes.
刊物名称: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
英文刊物名称: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
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学科: Medicine, Research & Experimental
英文学科: Medicine, Research & Experimental
影响因子: 2.341
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论文类别: Article
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