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论文题目: Simultaneous determination of amitraz, chlordimeform, formetanate and their main metabolites in human urine by high performance liquid chromatography-tandem mass spectrometry
英文论文题目: Simultaneous determination of amitraz, chlordimeform, formetanate and their main metabolites in human urine by high performance liquid chromatography-tandem mass spectrometry
第一作者: Gao, X; Tan, YL; Guo, H
英文第一作者: Gao, X; Tan, YL; Guo, H
联系作者: Guo, H (reprint author), Chongqing Inst Forens Sci, Chongqing 400021, Peoples R China.
英文联系作者: Guo, H (reprint author), Chongqing Inst Forens Sci, Chongqing 400021, Peoples R China.
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发表年度: 2017
卷: 1052
期:
页码: 27-33
摘要: A rapid, simple and reliable high performance liquid chromatography -tandem mass spectrometry (HPLC MS/MS) method was developed for simultaneous determination of amitraz, chlordimeform, formetanate and their main metabolites, N-(2,4-dimethylphenyl)-N-methyl-formamidine (DMPF), 2,4-dimethylformamidine (DMF), 2,4-dimethylaniline (DMA), 4-chloro-2-methylaniline and 3hydroxyacetanilide in human urine. The urine samples were mixed with buffer solutions (pH 8) and subsequently cleaned up by solid supported liquid/liquid extraction (SLE). The target analytes were efficiently separated with a Waters Atlantis T3 column (150 mm x 4.6 mm, 5 mu m), ionized with electrospray ion source in positive mode, and quantitatively determined by tandem mass spectrometry in the multiple reaction monitoring (MRM) mode. In order to minimize matrix effects, the matrix -matched calibration curves of eight analytes were adopted with correlation coefficients (R-2) above 0.99. The method were further validated by determining the limits of detection (LODs, 0.3-0.6 ng/mL), the limits of quantitation (LOQs, 1.0-2.0 ng/mL) and recoveries (89.1%-108.4%) with intra-day and inter -day relative standard deviation (RSD, <11%). The established method was applied and demonstrated in a real case by assaying a urine sample from a female poisoned by formetanate. The achieved results proved this method to be rapid, sensitive and accurate for simultaneous quantitation of eight analytes in human urine for intended forensic cases of human poisoning.
英文摘要: A rapid, simple and reliable high performance liquid chromatography -tandem mass spectrometry (HPLC MS/MS) method was developed for simultaneous determination of amitraz, chlordimeform, formetanate and their main metabolites, N-(2,4-dimethylphenyl)-N-methyl-formamidine (DMPF), 2,4-dimethylformamidine (DMF), 2,4-dimethylaniline (DMA), 4-chloro-2-methylaniline and 3hydroxyacetanilide in human urine. The urine samples were mixed with buffer solutions (pH 8) and subsequently cleaned up by solid supported liquid/liquid extraction (SLE). The target analytes were efficiently separated with a Waters Atlantis T3 column (150 mm x 4.6 mm, 5 mu m), ionized with electrospray ion source in positive mode, and quantitatively determined by tandem mass spectrometry in the multiple reaction monitoring (MRM) mode. In order to minimize matrix effects, the matrix -matched calibration curves of eight analytes were adopted with correlation coefficients (R-2) above 0.99. The method were further validated by determining the limits of detection (LODs, 0.3-0.6 ng/mL), the limits of quantitation (LOQs, 1.0-2.0 ng/mL) and recoveries (89.1%-108.4%) with intra-day and inter -day relative standard deviation (RSD, <11%). The established method was applied and demonstrated in a real case by assaying a urine sample from a female poisoned by formetanate. The achieved results proved this method to be rapid, sensitive and accurate for simultaneous quantitation of eight analytes in human urine for intended forensic cases of human poisoning.
刊物名称: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
英文刊物名称: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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学科: Biochemical Research Methods; Chemistry, Analytical
英文学科: Biochemical Research Methods; Chemistry, Analytical
影响因子: 2.603
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论文类别: Article
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