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论文题目: Individual and Combined Cytotoxic Effects of Co-Occurring Deoxynivalenol Family Mycotoxins on Human Gastric Epithelial Cells
英文论文题目: Individual and Combined Cytotoxic Effects of Co-Occurring Deoxynivalenol Family Mycotoxins on Human Gastric Epithelial Cells
第一作者: Yang, YX; Yu, S; Tan, YL; Liu, N; Wu, AB
英文第一作者: Yang, YX; Yu, S; Tan, YL; Liu, N; Wu, AB
联系作者: Wu, AB (reprint author), Univ Chinese Acad Sci, SIBS UGENT SJTU Joint Lab Mycotoxin Res, Key Lab Food Safety Res, Inst Nutr Sci,Shanghai Inst Biol Sci,Chinese Acad, Shanghai 200000, Peoples R China.
英文联系作者: Wu, AB (reprint author), Univ Chinese Acad Sci, SIBS UGENT SJTU Joint Lab Mycotoxin Res, Key Lab Food Safety Res, Inst Nutr Sci,Shanghai Inst Biol Sci,Chinese Acad, Shanghai 200000, Peoples R China.
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发表年度: 2017
卷: 9
期: 3
页码: 96
摘要: Mycotoxin contamination is a significant health concern for human beings, but health risk assessments are usually based on one single mycotoxin, which might neglect the additive or competitive interactions between co-occurring mycotoxins. In this study, we assessed the individual or combined toxicological effects to multiple deoxynivalenol-family mycotoxins, namely deoxynivalenol (DON), Nivalenol (NIV), and their acetyl derivatives of 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), deoxynivalenol-3-glucoside (D3G), and Fusarenon-X (FX) based on the human gastric epithelial (GES-1) cells. GES-1 cells were treated at different concentrations over 24 h and cell viability was measured by a cell counting kit (CCK8). The results show that D3G has no toxicity and 3-ADON is less potent in reducing cell viability compared to DON, whereas 15-ADON and FX appear to be slightly less potent than their parent compounds of DON and NIV on GES-1 cells. In general, the toxic ability of individual mycotoxins was shown as 3-ADON << 15-ADON < DON < FX < NIV, in an increasing order. All mixtures caused a dose-dependent decline of cell viability and the interactions analysis of binary combinations were assessed using the combination index (CI)-isobologram method. For the interaction types of mycotoxins mixtures, the synergistic cytotoxicity of DON + 15-ADON, DON + NIV, and DON + FX at low and/or moderate inhibitory concentration levels (IC10-IC70, IC10-IC80, and IC10-IC40, respectively) were observed. FX + NIV resulted in almost completely synergistic cytotoxicity, whereas 15-ADON + NIV and 15-ADON + FX presented almost entirely antagonistic cytotoxicity on the GES-1 cell model. These results suggest that the simultaneous presence of low-dose type B trichothecenes in dietary food may be more or less toxic than the prediction based on individual mycotoxins.
英文摘要: Mycotoxin contamination is a significant health concern for human beings, but health risk assessments are usually based on one single mycotoxin, which might neglect the additive or competitive interactions between co-occurring mycotoxins. In this study, we assessed the individual or combined toxicological effects to multiple deoxynivalenol-family mycotoxins, namely deoxynivalenol (DON), Nivalenol (NIV), and their acetyl derivatives of 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), deoxynivalenol-3-glucoside (D3G), and Fusarenon-X (FX) based on the human gastric epithelial (GES-1) cells. GES-1 cells were treated at different concentrations over 24 h and cell viability was measured by a cell counting kit (CCK8). The results show that D3G has no toxicity and 3-ADON is less potent in reducing cell viability compared to DON, whereas 15-ADON and FX appear to be slightly less potent than their parent compounds of DON and NIV on GES-1 cells. In general, the toxic ability of individual mycotoxins was shown as 3-ADON << 15-ADON < DON < FX < NIV, in an increasing order. All mixtures caused a dose-dependent decline of cell viability and the interactions analysis of binary combinations were assessed using the combination index (CI)-isobologram method. For the interaction types of mycotoxins mixtures, the synergistic cytotoxicity of DON + 15-ADON, DON + NIV, and DON + FX at low and/or moderate inhibitory concentration levels (IC10-IC70, IC10-IC80, and IC10-IC40, respectively) were observed. FX + NIV resulted in almost completely synergistic cytotoxicity, whereas 15-ADON + NIV and 15-ADON + FX presented almost entirely antagonistic cytotoxicity on the GES-1 cell model. These results suggest that the simultaneous presence of low-dose type B trichothecenes in dietary food may be more or less toxic than the prediction based on individual mycotoxins.
刊物名称: TOXINS
英文刊物名称: TOXINS
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学科: Toxicology
英文学科: Toxicology
影响因子: 3.03
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论文类别: Article
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