论文库首页  论文库
 
论文编号:
论文题目: Interactions of human embryonic stem cell-derived cardiovascular progenitor cells with immobilized extracellular matrix proteins
英文论文题目: Interactions of human embryonic stem cell-derived cardiovascular progenitor cells with immobilized extracellular matrix proteins
第一作者: Lu, JZ; Kaestle, K; Huang, JJ; Liu, Q; Zhang, P; Gao, L; Gardiner, J; Thissen, H; Yang, HT
英文第一作者: Lu, JZ; Kaestle, K; Huang, JJ; Liu, Q; Zhang, P; Gao, L; Gardiner, J; Thissen, H; Yang, HT
联系作者: Yang, HT (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Key Lab Stem Cell Biol, Biol Res Bldg A,320 Yueyang Rd, Shanghai 200031, Peoples R China.
英文联系作者: Yang, HT (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Key Lab Stem Cell Biol, Biol Res Bldg A,320 Yueyang Rd, Shanghai 200031, Peoples R China.
外单位作者单位:
英文外单位作者单位:
发表年度: 2017
卷: 105
期: 4
页码: 1094-1104
摘要: Human embryonic stem cell-derived cardiovascular progenitor cells (hESC-CVPCs) hold great promise for cell-based therapies of heart diseases. However, little is known about their niche microenvironment and in particular the required extracellular matrix (ECM) components. Here we screened combinations of surface-immobilized ECM proteins to identify substrates that support the attachment and survival of hESC-CVPCs. Covalent immobilization of ECM proteins laminin (Lm), fibronectin (Fn), collagen I (CI), collagen III (CIII), and collagen IV (CIV) in multiple combinations and concentrations was achieved by reductive amination on transparent acetaldehyde plasma polymer (AAPP) interlayer coatings. We identified that CI, CIII, CIV, and Fn and their combinations were important for hESC-CVPC attachment and survival, while Lm was dispensable. Moreover, for coatings displaying single ECM proteins, CI and CIII performed better than CIV and Fn, while coatings displaying the combined ECM proteins CIII+CIV and Fn+CIII+CIV at 100 mu g/mL were comparable to Matrigel in regard to supporting hESC-CVPC attachment and viability. Our results identify ECM proteins required for hESC-CVPCs and demonstrate that coatings displaying multiple immobilized ECM proteins offer a suitable microenvironment for the attachment and survival of hESC-CVPCs. This knowledge contributes to the development of approaches for maintaining hESC-CVPCs and therefore to advances in cardiovascular regeneration.
英文摘要: Human embryonic stem cell-derived cardiovascular progenitor cells (hESC-CVPCs) hold great promise for cell-based therapies of heart diseases. However, little is known about their niche microenvironment and in particular the required extracellular matrix (ECM) components. Here we screened combinations of surface-immobilized ECM proteins to identify substrates that support the attachment and survival of hESC-CVPCs. Covalent immobilization of ECM proteins laminin (Lm), fibronectin (Fn), collagen I (CI), collagen III (CIII), and collagen IV (CIV) in multiple combinations and concentrations was achieved by reductive amination on transparent acetaldehyde plasma polymer (AAPP) interlayer coatings. We identified that CI, CIII, CIV, and Fn and their combinations were important for hESC-CVPC attachment and survival, while Lm was dispensable. Moreover, for coatings displaying single ECM proteins, CI and CIII performed better than CIV and Fn, while coatings displaying the combined ECM proteins CIII+CIV and Fn+CIII+CIV at 100 mu g/mL were comparable to Matrigel in regard to supporting hESC-CVPC attachment and viability. Our results identify ECM proteins required for hESC-CVPCs and demonstrate that coatings displaying multiple immobilized ECM proteins offer a suitable microenvironment for the attachment and survival of hESC-CVPCs. This knowledge contributes to the development of approaches for maintaining hESC-CVPCs and therefore to advances in cardiovascular regeneration.
刊物名称: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
英文刊物名称: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
论文全文:
英文论文全文:
全文链接:
其它备注:
英文其它备注:
学科: Engineering, Biomedical; Materials Science, Biomaterials
英文学科: Engineering, Biomedical; Materials Science, Biomaterials
影响因子: 3.076
第一作者所在部门:
英文第一作者所在部门:
论文出处:
英文论文出处:
论文类别: Article
英文论文类别: Article
参与作者:
英文参与作者:
 
2014 中国科学院上海生命科学研究院 版权所有