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论文题目: Immune response after autologous hematopoietic stem cell transplantation in type 1 diabetes mellitus
英文论文题目: Immune response after autologous hematopoietic stem cell transplantation in type 1 diabetes mellitus
第一作者: Ye, L; Li, L; Wan, B; Yang, ML; Hong, J; Gu, WQ; Wang, WQ; Ning, G
英文第一作者: Ye, L; Li, L; Wan, B; Yang, ML; Hong, J; Gu, WQ; Wang, WQ; Ning, G
联系作者: Gu, WQ (reprint author), Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Endocrinol & Metab,Shanghai Inst Endocrine &, 197 Ruijin 2nd Rd, Shanghai 200025, Peoples R China.
英文联系作者: Gu, WQ (reprint author), Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Endocrinol & Metab,Shanghai Inst Endocrine &, 197 Ruijin 2nd Rd, Shanghai 200025, Peoples R China.
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发表年度: 2017
卷: 8
期:
页码: 90
摘要: Background: This study explored the details of the immune response after autologous hematopoietic stem cell transplantation (AHSCT) treatment in type 1 diabetes mellitus. Methods: Peripheral blood mononuclear cells (PBMCs) from 18 patients with type 1 diabetes mellitus were taken at baseline and 12 months after AHSCT or insulin-only therapy. The lymphocyte proliferation, mRNA expression and secretion of pro-inflammatory and anti-inflammatory cytokines belonging to T-helper type 1 (Th1), T-helper type 17 (Th17) and regulatory T (Treg) cells in PBMC culture supernatants were assessed. Results: Compared with patients receiving insulin-only treatment, the patients receiving AHSCT treatment showed better residual C-peptide secretion, lower anti-GAD titers and less exogenous insulin dosages after 12 months of follow-up. AHSCT treatment was associated with significantly reduced Th1 and Th17 cell proportions as well as decreased IFN-gamma, IL-2, IL-12p40 and IL-17A levels in the PBMC culture supernatants (all P < 0.05). Although there was no significant Treg cell expansion after AHSCT treatment, we observed increased IL-10, TGF-beta and Foxp3 mRNA expression and increased TGF-beta levels. However, we found no significant changes in the T-cell subpopulations after insulin treatment, except for higher IL-12p40 mRNA expression and a lower proportion of Treg cells. Conclusions: AHSCT treatment was associated with decreased expansion and function of Th1 and Th17 cells, which may explain the better therapeutic effect of AHSCT compared with the traditional intensive insulin therapy.
英文摘要: Background: This study explored the details of the immune response after autologous hematopoietic stem cell transplantation (AHSCT) treatment in type 1 diabetes mellitus. Methods: Peripheral blood mononuclear cells (PBMCs) from 18 patients with type 1 diabetes mellitus were taken at baseline and 12 months after AHSCT or insulin-only therapy. The lymphocyte proliferation, mRNA expression and secretion of pro-inflammatory and anti-inflammatory cytokines belonging to T-helper type 1 (Th1), T-helper type 17 (Th17) and regulatory T (Treg) cells in PBMC culture supernatants were assessed. Results: Compared with patients receiving insulin-only treatment, the patients receiving AHSCT treatment showed better residual C-peptide secretion, lower anti-GAD titers and less exogenous insulin dosages after 12 months of follow-up. AHSCT treatment was associated with significantly reduced Th1 and Th17 cell proportions as well as decreased IFN-gamma, IL-2, IL-12p40 and IL-17A levels in the PBMC culture supernatants (all P < 0.05). Although there was no significant Treg cell expansion after AHSCT treatment, we observed increased IL-10, TGF-beta and Foxp3 mRNA expression and increased TGF-beta levels. However, we found no significant changes in the T-cell subpopulations after insulin treatment, except for higher IL-12p40 mRNA expression and a lower proportion of Treg cells. Conclusions: AHSCT treatment was associated with decreased expansion and function of Th1 and Th17 cells, which may explain the better therapeutic effect of AHSCT compared with the traditional intensive insulin therapy.
刊物名称: STEM CELL RESEARCH & THERAPY
英文刊物名称: STEM CELL RESEARCH & THERAPY
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学科: Cell Biology; Medicine, Research & Experimental
英文学科: Cell Biology; Medicine, Research & Experimental
影响因子: 4.211
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论文类别: Article
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