论文库首页  论文库
 
论文编号:
论文题目: A class of circadian long non-coding RNAs mark enhancers modulating long-range circadian gene regulation
英文论文题目: A class of circadian long non-coding RNAs mark enhancers modulating long-range circadian gene regulation
第一作者: Fan, ZH; Zhao, M; Joshi, PD; Li, P; Zhang, Y; Guo, WM; Xu, YC; Wang, HF; Zhao, ZH; Yan, J
英文第一作者: Fan, ZH; Zhao, M; Joshi, PD; Li, P; Zhang, Y; Guo, WM; Xu, YC; Wang, HF; Zhao, ZH; Yan, J
联系作者: Yan, J (reprint author), Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai Inst Biol Sci, Inst Neurosci,State Key Lab Neurosci, Shanghai 200031, Peoples R China.
英文联系作者: Yan, J (reprint author), Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai Inst Biol Sci, Inst Neurosci,State Key Lab Neurosci, Shanghai 200031, Peoples R China.
外单位作者单位:
英文外单位作者单位:
发表年度: 2017
卷: 45
期: 10
页码: 5720-5738
摘要: Circadian rhythm exerts its influence on animal physiology and behavior by regulating gene expression at various levels. Here we systematically explored circadian long non-coding RNAs (lncRNAs) in mouse liver and examined their circadian regulation. We found that a significant proportion of circadian lncRNAs are expressed at enhancer regions, mostly bound by two key circadian transcription factors, BMAL1 and REV-ERB alpha. These circadian lncRNAs showed similar circadian phases with their nearby genes. The extent of their nuclear localization is higher than protein coding genes but less than enhancer RNAs. The association between enhancer and circadian lncRNAs is also observed in tissues other than liver. Comparative analysis between mouse and rat circadian liver transcriptomes showed that circadian transcription at lncRNA loci tends to be conserved despite of low sequence conservation of lncRNAs. One such circadian lncRNA termed lnc-Crot led us to identify a super-enhancer region interacting with a cluster of genes involved in circadian regulation of metabolism through long-range interactions. Further experiments showed that lnc-Crot locus has enhancer function independent of lnc-Crot's transcription. Our results suggest that the enhancer-associated circadian lncRNAs mark the genomic loci modulating long-range circadian gene regulation and shed new lights on the evolutionary origin of lncRNAs.
英文摘要: Circadian rhythm exerts its influence on animal physiology and behavior by regulating gene expression at various levels. Here we systematically explored circadian long non-coding RNAs (lncRNAs) in mouse liver and examined their circadian regulation. We found that a significant proportion of circadian lncRNAs are expressed at enhancer regions, mostly bound by two key circadian transcription factors, BMAL1 and REV-ERB alpha. These circadian lncRNAs showed similar circadian phases with their nearby genes. The extent of their nuclear localization is higher than protein coding genes but less than enhancer RNAs. The association between enhancer and circadian lncRNAs is also observed in tissues other than liver. Comparative analysis between mouse and rat circadian liver transcriptomes showed that circadian transcription at lncRNA loci tends to be conserved despite of low sequence conservation of lncRNAs. One such circadian lncRNA termed lnc-Crot led us to identify a super-enhancer region interacting with a cluster of genes involved in circadian regulation of metabolism through long-range interactions. Further experiments showed that lnc-Crot locus has enhancer function independent of lnc-Crot's transcription. Our results suggest that the enhancer-associated circadian lncRNAs mark the genomic loci modulating long-range circadian gene regulation and shed new lights on the evolutionary origin of lncRNAs.
刊物名称: NUCLEIC ACIDS RESEARCH
英文刊物名称: NUCLEIC ACIDS RESEARCH
论文全文:
英文论文全文:
全文链接:
其它备注:
英文其它备注:
学科: Biochemistry & Molecular Biology
英文学科: Biochemistry & Molecular Biology
影响因子: 10.162
第一作者所在部门:
英文第一作者所在部门:
论文出处:
英文论文出处:
论文类别: Article
英文论文类别: Article
参与作者:
英文参与作者:
 
2014 中国科学院上海生命科学研究院 版权所有