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论文题目: Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging
英文论文题目: Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging
第一作者: Liu, MM; Li, Q; Liang, L; Li, J; Wang, K; Li, JJ; Lv, M; Chen, N; Song, HY; Lee, J; Shi, JY; Wang, LH; Lal, R; Fan, CH
英文第一作者: Liu, MM; Li, Q; Liang, L; Li, J; Wang, K; Li, JJ; Lv, M; Chen, N; Song, HY; Lee, J; Shi, JY; Wang, LH; Lal, R; Fan, CH
联系作者: Fan, CH (reprint author), Chinese Acad Sci, Div Phys Biol, Shanghai 201800, Peoples R China.
英文联系作者: Fan, CH (reprint author), Chinese Acad Sci, Div Phys Biol, Shanghai 201800, Peoples R China.
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发表年度: 2017
卷: 8
期:
页码: 15646
摘要: Mechanistic understanding of the endocytosis and intracellular trafficking of nanoparticles is essential for designing smart theranostic carriers. Physico-chemical properties, including size, clustering and surface chemistry of nanoparticles regulate their cellular uptake and transport. Significantly, even single nanoparticles could cluster intracellularly, yet their clustering state and subsequent trafficking are not well understood. Here, we used DNA-decorated gold (fPlas-gold) nanoparticles as a dually emissive fluorescent and plasmonic probe to examine their clustering states and intracellular transport. Evidence from correlative fluorescence and plasmonic imaging shows that endocytosis of fPlas-gold follows multiple pathways. In the early stages of endocytosis, fPlas-gold nanoparticles appear mostly as single particles and they cluster during the vesicular transport and maturation. The speed of encapsulated fPlas-gold transport was critically dependent on the size of clusters but not on the types of organelle such as endosomes and lysosomes. Our results provide key strategies for engineering theranostic nanocarriers for efficient health management.
英文摘要: Mechanistic understanding of the endocytosis and intracellular trafficking of nanoparticles is essential for designing smart theranostic carriers. Physico-chemical properties, including size, clustering and surface chemistry of nanoparticles regulate their cellular uptake and transport. Significantly, even single nanoparticles could cluster intracellularly, yet their clustering state and subsequent trafficking are not well understood. Here, we used DNA-decorated gold (fPlas-gold) nanoparticles as a dually emissive fluorescent and plasmonic probe to examine their clustering states and intracellular transport. Evidence from correlative fluorescence and plasmonic imaging shows that endocytosis of fPlas-gold follows multiple pathways. In the early stages of endocytosis, fPlas-gold nanoparticles appear mostly as single particles and they cluster during the vesicular transport and maturation. The speed of encapsulated fPlas-gold transport was critically dependent on the size of clusters but not on the types of organelle such as endosomes and lysosomes. Our results provide key strategies for engineering theranostic nanocarriers for efficient health management.
刊物名称: NATURE COMMUNICATIONS
英文刊物名称: NATURE COMMUNICATIONS
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学科: Multidisciplinary Sciences
英文学科: Multidisciplinary Sciences
影响因子: 12.124
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论文类别: Article
英文论文类别: Article
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