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论文题目: 3D Chromatin Structures of Mature Gametes and Structural Reprogramming during Mammalian Embryogenesis
英文论文题目: 3D Chromatin Structures of Mature Gametes and Structural Reprogramming during Mammalian Embryogenesis
第一作者: Ke, YW; Xu, YA; Chen, XP; Feng, SK; Liu, ZB; Sun, YY; Yao, XL; Li, FZ; Zhu, W; Gao, L; Chen, HJ; Du, ZH; Xie, W; Xu, XC; Huang, XX; Liu, J
英文第一作者: Ke, YW; Xu, YA; Chen, XP; Feng, SK; Liu, ZB; Sun, YY; Yao, XL; Li, FZ; Zhu, W; Gao, L; Chen, HJ; Du, ZH; Xie, W; Xu, XC; Huang, XX; Liu, J
联系作者: Liu, J (reprint author), Chinese Acad Sci, Beijing Inst Genom, Collaborat Innovat Ctr Genet & Dev, CAS Key Lab Genome Sci & Informat, Beijing, Peoples R China.
英文联系作者: Liu, J (reprint author), Chinese Acad Sci, Beijing Inst Genom, Collaborat Innovat Ctr Genet & Dev, CAS Key Lab Genome Sci & Informat, Beijing, Peoples R China.
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发表年度: 2017
卷: 170
期: 2
页码: 367-+
摘要: High-order chromatin structure plays important roles in gene expression regulation. Knowledge of the dynamics of 3D chromatin structures during mammalian embryo development remains limited. We report the 3D chromatin architecture of mouse gametes and early embryos using an optimized Hi-C method with low-cell samples. We find that mature oocytes at the metaphase II stage do not have topologically associated domains (TADs). In sperm, extra-long-range interactions (>4 Mb) and interchromosomal interactions occur frequently. The high-order structures of both the paternal and maternal genomes in zygotes and two-cell embryos are obscure but are gradually re-established through development. The establishment of the TAD structure requires DNA replication but not zygotic genome activation. Furthermore, unmethylated CpGs are enriched in A compartment, and methylation levels are decreased to a greater extent in A compartment than in B compartment in embryos. In summary, the global reprogramming of chromatin architecture occurs during early mammalian development.
英文摘要: High-order chromatin structure plays important roles in gene expression regulation. Knowledge of the dynamics of 3D chromatin structures during mammalian embryo development remains limited. We report the 3D chromatin architecture of mouse gametes and early embryos using an optimized Hi-C method with low-cell samples. We find that mature oocytes at the metaphase II stage do not have topologically associated domains (TADs). In sperm, extra-long-range interactions (>4 Mb) and interchromosomal interactions occur frequently. The high-order structures of both the paternal and maternal genomes in zygotes and two-cell embryos are obscure but are gradually re-established through development. The establishment of the TAD structure requires DNA replication but not zygotic genome activation. Furthermore, unmethylated CpGs are enriched in A compartment, and methylation levels are decreased to a greater extent in A compartment than in B compartment in embryos. In summary, the global reprogramming of chromatin architecture occurs during early mammalian development.
刊物名称: CELL
英文刊物名称: CELL
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学科: Biochemistry & Molecular Biology; Cell Biology
英文学科: Biochemistry & Molecular Biology; Cell Biology
影响因子: 30.41
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论文类别: Article
英文论文类别: Article
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