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姓 名:
佘益民
性    别:
专家类别:
研究员
学 历:
博士
所属部门:
上海植物逆境生物学研究中心
学科类别:
电 话:
传 真:
电子邮件:
ymshe@sibs.ac.cn
通讯地址:
中国上海市松江区辰花路3888号

简历:
1994-1997年,中国科学院长春应用化学学院,获博士学位;
1997-2001年,加拿大马尼托巴大学,物理系博士后研究;
2001-2007年,加拿大多伦多大学儿童医院蛋白质组学实验室负责人;
2007-2012年,加拿大皇后大学化学系质谱和蛋白质组学实验室负责人、兼职教授、研究科学家;
2012年7月-今,中科院上海逆境生物学研究中心担任研究员、蛋白质组学平台负责人。

研究方向:
蛋白质与代谢组学
The laboratory utilizes chromatography and mass spectrometry to investigate 1) protein post-translational modifications and structure-function aspects involved in plant growth and development; 2) proteins that help plants acclimate to environmental stresses such as drought, salinity, temperature extremes, and nutrient limitation; and 3) protein-protein interactions and biological pathways that mediate plant defence to bacterial and viral pathogens. We aim to establish existing proteomics methods and develop newly advanced techniques together with additional scientific resources to meet the demands of cutting-edge plant science and biomedical research. 
Research efforts focus on the large-scale identification and quantification of proteins (i.e. proteome) extracted from plant cells and tissues, particularly those post-translationally modified by phosphorylation, glycosylation, ubiquitination and acetylation. Such analyses will localize the modifications on protein three-dimensional structural domains, and help to establish their influence on the activity, stability, degradation and conformation of plant enzymes, and to determine the regulatory role relevant to plant metabolism, growth and development. Through studying protein-protein interactions, we also develop quantitative proteomics strategies to resolve the signaling pathways of viral infection, proliferation, interaction, and immunization in host cells, which will ultimately develop plant varieties that are highly resistant to diseases.

职称:

职务:

社会任职:

获奖及荣誉:
代表论著:
 1. She YM, Rosu-Myles M, Walrond L, Cyr TD. Quantifying protein isoforms in Mesenchymal stem cells by reductive dimethylation of lysines in intact proteins. Proteomics. 2012, 12(3), 369-379.
2. Visser-Grieve S, Zhou Z, She YM, Huang H, Cyr TD, Xu T, Yang X. LATS1 tumor suppressor is a novel actin-binding protein and negative regulator of actin polymerization. Cell Res. 2011, 21(10), 1513-1516. 
3. Ho KC, Zhou Z, She YM, Cyr TD, Yang X. Itch E3 ubiquitin ligase regulates large tumor suppressor 1 stability. Proc. Natl. Acad. Sci. USA, 2011, 108(12), 4870-4875. 
4. She YM, Xu X, Yakunin AF, Dhe-Paganon S, Donald LJ, Standing KG, Lee D, Jia Z, Cyr TD. Mass spectrometry following mild enzymatic digestion reveals phosphorylation of recombinant proteins in Escherichia coli through mechanisms involving direct nucleotide binding. J. Proteome Res. 2010. 9(6), 3311-3318.
5. Fouladkou F, Lu C, Jiang C, Zhou L, She YM, Walls JR, Kawabe H, Brose N, Henkelman RM, Huang A, Bruneau BG, Rotin D. The ubiquitin ligase NEDD4-1 is required for heart development and is a suppressor of thrombospondin-1. J Biol Chem. 2010, 285(9), 6770-6780.
6. Lee DC, Zheng J, She YM, Jia Z. Structure of Escherichia coli tyrosine kinase EtK reveals novel activation mechanism. EMBO J. 2008, 27(12), 1758-1766. 
7. Uhrig RG, She YM, Leach CA, Plaxton WC. Regulatory monoubiquitination of phosphoenolypyruvate carboxylase. J. Biol. Chem. 2008, 283(44), 29650-29657. 
8. She YM, Krokhin O, Spicer V, Loboda A, Garland G, Ens W, Standing KG. Formation of (bn-1 + H2O) ions by collisional activation of MALDI-formed peptide [M+H]+ ions in a QqTOF mass spectrometer. J. Am. Soc. Mass Spectrom. 2007, 18(6), 1024-1037. 
9. Weerasekera R, She YM, Markham K, Bai Y, Opalka N, Orlicky S, Sicheri F, Schmitt-Ulms G. Interactome and interface protocol (2IP): a novel strategy for high sensitivity topology mapping of protein complexes. Proteomics, 2007, 7(21), 3835-3852.
10. Smith CA, Lau KM, Rahmani Z, Dho SE, Brothers G, She YM, Berry DM, Bonneil E, Thibault P, Schweisguth F, Le Borgne R, McGlade CJ. aPKC-mediated phosphorylation regulates asymmetric membrane localization of the cell fate determinant Numb. EMBO J. 2007, 26 (2), 468-480. 
11. Ramjeesingh M., Li C., She YM, Bear C. Evaluation of the membrane spanning domain of ClC-2. Biochem. J. 2006. 396 (3), 449-460. 
12. She YM, Seifers DL, Haber S, Ens W, Standing KG. Characterization of the agent of “High Plains Disease”: mass spectrometry determines the sequence of the disease-specific protein. J. Biol. Chem. 2004, 279(1), 488-494.
13. She YM, Huang YW, Zhang L, Trimble WS. Septin 2 phosphorylation: theoretical and mass spectrometric evidence for the existence of a single phosphorylation site in vivo. Rapid Commun. Mass Spectrom. 2004, 18(10), 1123-1130.
14. She YM, Narindrasorasak S, Yang S, Spitale N, Roberts EA, Sarkar B. Identification of metal-binding proteins in human hepatoma lines by immobilized metal affinity chromatography and mass spectrometry. Mol. Cell Proteomics. 2003, 2(12), 1306-1318.
15. She YM, Wang GQ, Loboda A, Ens W, Standing KG, Burczynski FJ. Sequencing rat liver cytosolic proteins by MALDI QqTOF mass spectrometry, following electrophoresis separation and extraction, Anal. Biochem. 2002, 310(2), 137-147.
16. Fu LH, Wang XF, Eyal Y, She YM, Donald LJ, Standing KG, Ben-Hayyim G. A selenoprotein in the plant kingdom: mass spectrometry confirms that an opal codon (UGA) encodes selenocysteine in Chlamydomonas reinhardtii glutathione peroxidase. J. Biol. Chem. 2002, 277(29), 25983-25991.
17. She YM, Haber S, Seifers DL, Loboda A, Chernushevich I, Perreault H, Ens W, Standing KG. Determination of the complete amino acid sequence for the coat protein of brome mosaic virus by time-of-flight mass spectrometry: evidence for mutations associated with change of propagation host. J. Biol. Chem. 2001, 276(23), 20039-20047.
18. She YM, Liu SY, Li XM. Ion/neutral complex-mediated reactions generated from some ionized tetrahydro imidazole-substituted methylene -diketones. Rapid Commun. Mass Spectrom. 1997, 11, 723-726.
19. She YM, Tu YP, Liu SY. C118 from fullerenols: formation, structure and intermolecular nC2 transfer reactions in mass spectrometry. Rapid Commun. Mass Spectrom. 1996, 10, 676-678.
20. She YM, Tu YP, Liu SY. Collision-induced fragmentation of ionized 3-phenyl-butyn-3-ol mediated by an intermediate proton-bound complex. J. Mass Spectrom. 1996, 31, 1191-1192.

承担科研项目情况:
 
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